流感血凝素介导含聚乙二醇接枝脂质的膜融合：对融合机制的新见解。

Influenza hemagglutinin mediated fusion of membranes containing poly(ethylene-glycol) grafted lipids: new insights into the fusion mechanism.

作者信息

Chams V, Bonnafous P, Stegmann T

机构信息

Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, Toulouse, France.

出版信息

FEBS Lett. 1999 Apr 1;448(1):28-32. doi: 10.1016/s0014-5793(99)00333-6.

DOI:10.1016/s0014-5793(99)00333-6

PMID:10217403

Abstract

The influence of a hydrophilic layer covering the membrane on influenza hemagglutinin (HA) mediated fusion was investigated using membranes containing poly(ethylene-glycol) grafted phosphatidylethanolamine (PEG-2000-PE). Steric inhibition of HA-membrane interactions by these lipids affected virus fusion (half-maximal inhibition at 0.8 mol% for lipids with 114 ethylene glycol residues, or at 3.2 mol% for 45 residues (PEG-2000-PE), concentrations at which the PEG moieties adopt a random coil structure). Reconstituted viral membranes containing 3 mol% PEG-2000-PE retained 40% of their fusion activity. Therefore, efficient fusion is possible with membranes completely covered by a hydrophilic layer of several nanometers, and fusogenic virosomes containing PEG-PE are feasible.

摘要

使用含有聚乙二醇接枝磷脂酰乙醇胺（PEG - 2000 - PE）的膜，研究了覆盖在膜上的亲水层对流感血凝素（HA）介导的融合的影响。这些脂质对HA - 膜相互作用的空间位阻影响了病毒融合（对于具有114个乙二醇残基的脂质，在0.8 mol%时达到半数最大抑制；对于45个残基的脂质（PEG - 2000 - PE），在3.2 mol%时达到半数最大抑制，此时PEG部分呈无规卷曲结构）。含有3 mol% PEG - 2000 - PE的重组病毒膜保留了其40%的融合活性。因此，用几纳米厚的亲水层完全覆盖的膜实现高效融合是可能的，并且含有PEG - PE的融合病毒体是可行的。