Kwak H J, So J N, Lee S J, Kim I, Koh G Y
National Creative Research Initiatives Center for Cardiac Regeneration and Institute of Cardiovascular Research, Chonbuk National University School of Medicine, Chonju, South Korea.
FEBS Lett. 1999 Apr 9;448(2-3):249-53. doi: 10.1016/s0014-5793(99)00378-6.
We examined the effect of angiopoietin-1 (Ang1) on apoptosis in human umbilical vein endothelial cells (HUVECs). Ang1 (5-1000 ng/ml) dose-dependently inhibited apoptosis under a serum-deprived state. A significant apoptotic inhibition occurred with as low as 50 ng/ml. Two hundred ng/ml of Ang1 inhibited to approximately 50% of the control apoptotic rates for 96 h. Furthermore, an augmented antiapoptotic effect of Ang1 by the addition of 20 ng/ml vascular endothelial growth factor was observed. This Ang1-induced strong antiapoptotic effect in endothelial cells is a novel and intriguing finding and could be an additional description of Ang1-induced direct biological function.
我们研究了血管生成素-1（Ang1）对人脐静脉内皮细胞（HUVECs）凋亡的影响。在血清剥夺状态下，Ang1（5 - 1000 ng/ml）呈剂量依赖性抑制细胞凋亡。低至50 ng/ml时就出现了显著的凋亡抑制。200 ng/ml的Ang1在96小时内将凋亡率抑制至对照组的约50%。此外，观察到添加20 ng/ml血管内皮生长因子可增强Ang1的抗凋亡作用。Ang1在内皮细胞中诱导的这种强大抗凋亡作用是一个新颖且有趣的发现，可能是对Ang1诱导的直接生物学功能的又一描述。
