Tanhauser S M, Yowell C A, Cutler T J, Greiner E C, MacKay R J, Dame J B
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville 32611-0880, USA.
J Parasitol. 1999 Apr;85(2):221-8.
Studies designed to investigate the causative agent of equine protozoal myeloencephalitis and its life cycle have been hampered by the marked similarity of Sarcocystis neurona to other Sarcocystis spp. present in the same definitive host. Random-amplified polymorphic DNA techniques were used to amplify DNA from isolates of S. neurona and Sarcocystis falcatula. DNA sequence analysis of polymerase chain reaction (PCR) products was then used to design PCR primers to amplify specific Sarcocystis spp. DNA products. The ribosomal RNA internal transcribed spacer was also amplified and compared between S. neurona and S. falcatula. Useful sequence heterogeneity between the 2 organisms was identified, creating potential markers to distinguish these Sarcocystis spp. These markers were used to characterize Sarcocystis isolates from opossum (Didelphis virginiana) feces. Our data suggest that S. neurona and S. falcatula can be differentiated with these markers and that multiple Sarcocystis spp., including S. neurona and S. falcatula, are shed by opossums.
旨在研究马属动物原虫性脑脊髓炎病原体及其生命周期的研究，因神经肉孢子虫与同一终末宿主体内存在的其他肉孢子虫属极为相似而受阻。采用随机扩增多态性DNA技术从神经肉孢子虫和镰状肉孢子虫分离株中扩增DNA。然后对聚合酶链反应（PCR）产物进行DNA序列分析，以设计PCR引物来扩增特定肉孢子虫属的DNA产物。还扩增了核糖体RNA内部转录间隔区，并在神经肉孢子虫和镰状肉孢子虫之间进行比较。在这两种生物体之间鉴定出有用的序列异质性，从而产生了区分这些肉孢子虫属的潜在标记。这些标记用于鉴定来自负鼠（弗吉尼亚负鼠）粪便的肉孢子虫分离株。我们的数据表明，利用这些标记可以区分神经肉孢子虫和镰状肉孢子虫，并且负鼠会排出包括神经肉孢子虫和镰状肉孢子虫在内的多种肉孢子虫属。
