Delecluse H J, Pich D, Hilsendegen T, Baum C, Hammerschmidt W
GSF-National Research Center for Environment and Health, Institute of Clinical Molecular Biology and Tumor Genetics, Department Gene Vectors, Marchioninistrasse 25, D-81377 Munich, Germany.
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5188-93. doi: 10.1073/pnas.96.9.5188.
On the basis of the B lymphotropic Epstein-Barr virus (EBV), we have constructed a virus-free packaging cell line that allows encapsidation of plasmids into herpesvirus particles. This cell line harbors an EBV mutant whose packaging signals have been deleted. The gene vectors, which can encompass very large, contiguous pieces of foreign DNA, carry all cis-acting elements involved in amplification and encapsidation into virus-like particles as well as those essential for extrachromosomal maintenance in the recipient cell. Although this first-generation packaging cell line suffers from unwanted recombination between the helper virus genome and gene vector DNAs, this approach opens the way to delivery and stable maintenance of any transgene in human B cells.
基于嗜B淋巴细胞的爱泼斯坦-巴尔病毒（EBV），我们构建了一种无病毒包装细胞系，该细胞系可将质粒包装到疱疹病毒颗粒中。此细胞系含有一个EBV突变体，其包装信号已被删除。基因载体可包含非常大的连续外源DNA片段，携带所有参与扩增和包装到病毒样颗粒中的顺式作用元件，以及受体细胞中染色体外维持所必需的元件。尽管这种第一代包装细胞系存在辅助病毒基因组与基因载体DNA之间不必要的重组问题，但这种方法为在人B细胞中递送和稳定维持任何转基因开辟了道路。
