Sharma A, Honma K, Sojar H T, Hruby D E, Kuramitsu H K, Genco R J
School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York, 14214, USA.
Biochem Biophys Res Commun. 1999 Apr 29;258(1):222-6. doi: 10.1006/bbrc.1999.0616.
Porphyromonas gingivalis, a gram-negative oral anaerobic bacterium, has been implicated in the onset and development of periodontitis. The P. gingivalis fimbriae which mediate bacterial adherence to host oral sites and induce host inflammatory responses have been suggested as a potential antigen candidate. for vaccine development. This study was undertaken to generate Streptococcus gordonii vectors expressing the major subunit protein (FimA) of P. gingivalis fimbriae for testing as a potential live vaccine against periodontitis. We report here the expression of the C-terminal saliva-binding epitopes of P. gingivalis FimA on the surface of S. gordonii and demonstrate that domains containing free cysteine residues are poorly expressed on the surface of S. gordonii.
牙龈卟啉单胞菌是一种革兰氏阴性口腔厌氧菌，与牙周炎的发生和发展有关。介导细菌黏附于宿主口腔部位并诱导宿主炎症反应的牙龈卟啉单胞菌菌毛，已被认为是一种潜在的疫苗开发抗原候选物。本研究旨在构建表达牙龈卟啉单胞菌菌毛主要亚基蛋白（FimA）的戈登链球菌载体，作为预防牙周炎的潜在活疫苗进行测试。我们在此报告牙龈卟啉单胞菌FimA的C端唾液结合表位在戈登链球菌表面的表达，并证明含有游离半胱氨酸残基的结构域在戈登链球菌表面表达不佳。
