Sharma A, Sojar H T, Hruby D E, Kuramitsu H K, Genco R J
School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York 14214, USA.
Biochem Biophys Res Commun. 1997 Sep 18;238(2):313-6. doi: 10.1006/bbrc.1997.7306.
The fimbriae of Porphyromonas gingivalis plays an important role in the pathogenesis of periodontal disease. A structural subunit of the P. gingivalis fimbriae, fimbrillin, has been shown to promote adherence of the bacteria to host surfaces and also induce an immune response. Biologically active domains of fimbrillin responsible for adherence or eliciting immune responses have been determined. In a previous study, we engineered the human oral commensal organism Streptococcus gordonii to express such biologically active domains on the surface of the bacteria as a vaccine delivery system. In this study we report an alternative approach of secreting fimbrillin polypeptide domains into the medium by modification of the surface-expression system described earlier. Such recombinant S. gordonii, in addition to being a source for antigen presentation to trigger a protective immune response, may have the added advantage of directly blocking the fimbriae-mediated adherence of P. gingivalis to the oral cavity following implantation. This approach can also be utilized for secreting other biologically important therapeutic molecules on mucosal surfaces for modulating local microenvironments.
牙龈卟啉单胞菌的菌毛在牙周疾病的发病机制中起重要作用。牙龈卟啉单胞菌菌毛的一种结构亚基菌毛蛋白，已被证明可促进细菌黏附于宿主表面并诱导免疫反应。负责黏附或引发免疫反应的菌毛蛋白的生物活性结构域已被确定。在先前的一项研究中，我们对人类口腔共生菌戈登链球菌进行基因工程改造，使其在细菌表面表达此类生物活性结构域，作为一种疫苗递送系统。在本研究中，我们报告了一种通过对先前描述的表面表达系统进行改造，将菌毛蛋白多肽结构域分泌到培养基中的替代方法。这种重组戈登链球菌，除了作为引发保护性免疫反应的抗原呈递来源外，在植入后可能还具有直接阻断牙龈卟啉单胞菌菌毛介导的黏附于口腔的额外优势。这种方法还可用于在黏膜表面分泌其他具有重要生物学意义的治疗分子，以调节局部微环境。
