Andrzejewska E, Szkaradkiewicz A, Kaniasty M
Zakład Mikrobiologii Lekarskiej A.M. im. Karola Marcinkowskiego w Poznaniu.
Med Dosw Mikrobiol. 1998;50(3-4):197-205.
The studies aimed at analysing the resistance to some beta-lactam antibiotics among E. coli and K. pneumoniae clinical isolates and at evaluating. The extended spectrum of beta-lactamases (ESBL) production in the isolates. The analysis included 137 E. coli strains and 52 K. pneumoniae strains, isolated from hospitalized patients and out-patients treated in the first trimester of 1998. The strains were identified using the ATB computer system. Antibiotic sensitivity of the isolates was determined by disc-diffusion tests. ESBL production capacity of E. coli and K. pneumoniae strains was estimated by double-disc and ATB BLSA tests. Most of the analysed E. coli strains were found to exhibit significant sensitivity to compound penicillin preparations containing beta-lactam inhibitor (Augmentin, Tazocin) and to the third generation cefalosporins, in contrast, K. pneumoniae strains much more frequently were resistant to the drugs. Among the obtained isolates, 3 (2.2%) E. coli strains and 21 (40.4%) K. pneumoniae strains produced ESBL but all the isolates proved sensitive to imipenem. In evaluation of ESBL production-detecting tests, the double-disc test was found to be more reliable than ATB BLSA test.
这些研究旨在分析大肠杆菌和肺炎克雷伯菌临床分离株对某些β-内酰胺类抗生素的耐药性，并评估分离株中β-内酰胺酶（ESBL）的产生情况。分析包括1998年第一季度从住院患者和门诊患者中分离出的137株大肠杆菌和52株肺炎克雷伯菌。使用ATB计算机系统对菌株进行鉴定。通过纸片扩散试验测定分离株的抗生素敏感性。通过双纸片试验和ATB BLSA试验评估大肠杆菌和肺炎克雷伯菌菌株产生ESBL的能力。结果发现，大多数分析的大肠杆菌菌株对含β-内酰胺酶抑制剂的复方青霉素制剂（阿莫西林克拉维酸钾、他唑巴坦钠）和第三代头孢菌素表现出显著敏感性，相比之下，肺炎克雷伯菌菌株对这些药物的耐药性更为常见。在所获得的分离株中，3株（2.2%）大肠杆菌和21株（40.4%）肺炎克雷伯菌产生ESBL，但所有分离株对亚胺培南均敏感。在评估ESBL产生检测试验时，发现双纸片试验比ATB BLSA试验更可靠。
