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来自健康和患病马匹的马驹放线杆菌分离株的生化指纹图谱分析和核糖分型

Biochemical fingerprinting and ribotyping of isolates of Actinobacillus equuli from healthy and diseased horses.

作者信息

Sternberg S, Brändström B

机构信息

Department of Veterinary Microbiology, Swedish University of Agricultural Sciences (SLU), Uppsala.

出版信息

Vet Microbiol. 1999 Mar 31;66(1):53-65. doi: 10.1016/s0378-1135(98)00303-4.

DOI:10.1016/s0378-1135(98)00303-4
PMID:10223322
Abstract

A total of 112 isolates of Actinobacillus equuli, including both clinical isolates and isolates from the oral cavity of healthy horses, were included in this study. All isolates were ribotyped and 92 of the isolates were also typed biochemically, with the commercially available Pheneplate (PhP) system, which includes 48 different substrates. As expected, ribotyping was more sensitive than biochemical fingerprinting in detecting differences between the isolates. The correlation between the two methods used was poor. It was not possible to distinguish clinical isolates from normal flora isolates by either of the two methods used.

摘要

本研究共纳入112株马驹放线杆菌分离株,包括临床分离株和健康马匹口腔分离株。对所有分离株进行了核糖体分型,其中92株分离株还使用包含48种不同底物的市售Pheneplate(PhP)系统进行了生化分型。正如预期的那样,核糖体分型在检测分离株之间的差异方面比生化指纹图谱更敏感。所使用的两种方法之间的相关性较差。使用这两种方法中的任何一种都无法区分临床分离株和正常菌群分离株。

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