Uchida-Fujii Eri, Niwa Hidekazu, Kinoshita Yuta, Nukada Toshio
Microbiology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan.
J Vet Med Sci. 2019 Sep 3;81(9):1234-1237. doi: 10.1292/jvms.19-0192. Epub 2019 Jul 11.
Actinobacillus species are known to be pathogenic to horses. To clarify etiological agents of actinobacillosis in Japanese adult horses, 27 isolates from Japanese Thoroughbred racehorses putatively identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry as Actinobacillus were further identified by PCR of the A. equuli toxin gene, by CAMP test, and by 16S rRNA sequencing analysis. Actinobacillus equuli subsp. haemolyticus was isolated most frequently (16/27) and was related to respiratory infections. Actinobacillus equuli subsp. equuli (4/27) was isolated from chronic cases or concomitant with other bacterial infections. The remainder were A. pleuropneumoniae, unclassified Actinobacillus species and Pasteurella caballi. Actinobacillus equuli including subsp. haemolyticus and subsp. equuli were the species most frequently isolated from equine actinobacillosis in Japan.
放线杆菌属已知可对马匹致病。为明确日本成年马放线杆菌病的病原体，对27株从日本纯种赛马中分离出的菌株进行了进一步鉴定，这些菌株通过基质辅助激光解吸电离飞行时间质谱法初步鉴定为放线杆菌属，采用马驹放线杆菌毒素基因PCR、CAMP试验和16S rRNA测序分析进行进一步鉴定。溶血马驹放线杆菌分离频率最高（16/27），与呼吸道感染有关。马驹放线杆菌马驹亚种（4/27）从慢性病例中分离得到或与其他细菌感染同时存在。其余的是胸膜肺炎放线杆菌、未分类的放线杆菌属物种和巴氏驹杆菌。包括溶血亚种和马驹亚种在内的马驹放线杆菌是日本马放线杆菌病中最常分离出的菌种。
