Poulet P P, Duffaut D, Lodter J P
Department of Oral Biology, School of Dental Medicine, Paul Sabatier University, Toulouse, France.
J Clin Periodontol. 1999 Apr;26(4):261-3. doi: 10.1034/j.1600-051x.1999.260411.x.
The aim of the present investigation was to determine the susceptibility of Prevotella intermedia, Porphyromonas gingivalis, Fusobacterium and Peptostreptococcus micros to metronidazole in vitro. Two methods were applied on each isolated strain: agar dilution and epsilometer Etest. A total of fifty three wild test strains (13 P.intermedia, 14 P. gingivalis, 14 F.spp and 12 P.micros) were isolated from patients with periodontitis. The Etest appears to be a simple, rapid and reliable method for the metronidazole susceptibility testing. The results show that all P.intermedia, P.gingivalis and F.spp strains were susceptible to metronidazole. The mean values of minimal inhibitory concentration obtained with the agar dilution method were, respectively, 0.98 microg/ml, 0.122 microg/ml and 0.242 microg/ml. For P.micros, the minimal inhibitory concentration was of 12.14 microg/ml. Comparatively to break points, only 60% of P.micros strains seem to be susceptible, in vitro, to metronidazole. This study demonstrated the excellent activity of metronidazole against P.intermedia, P.gingivalis, F.spp except perhaps for P.micros.
本研究的目的是在体外确定中间普氏菌、牙龈卟啉单胞菌、梭杆菌和微小消化链球菌对甲硝唑的敏感性。对每个分离菌株应用了两种方法：琼脂稀释法和埃普利法（Etest）。从牙周炎患者中总共分离出53株野生测试菌株（13株中间普氏菌、14株牙龈卟啉单胞菌、14株梭杆菌属和12株微小消化链球菌）。埃普利法似乎是一种用于甲硝唑敏感性测试的简单、快速且可靠的方法。结果表明，所有中间普氏菌、牙龈卟啉单胞菌和梭杆菌属菌株对甲硝唑敏感。用琼脂稀释法获得的最低抑菌浓度平均值分别为0.98微克/毫升、0.122微克/毫升和0.242微克/毫升。对于微小消化链球菌，最低抑菌浓度为12.14微克/毫升。与断点相比，在体外只有60%的微小消化链球菌菌株似乎对甲硝唑敏感。本研究证明了甲硝唑对中间普氏菌、牙龈卟啉单胞菌、梭杆菌属具有优异的活性，也许微小消化链球菌除外。
