Ichimi R, Jin-no T, Ito M
Department of Pediatrics, Mie University School of Medicine, Tsu, Japan.
J Med Virol. 1999 May;58(1):63-8. doi: 10.1002/(sici)1096-9071(199905)58:1<63::aid-jmv10>3.0.co;2-c.
Apoptosis induced by human herpesvirus 6 (HHV-6) in cord blood lymphocytes was investigated. Cord blood mononuclear cells (CBMC) prestimulated with phytohemagglutinin (PHA) were infected with HHV-6 and cultured with interleukin 2 (IL-2) for 5 days. Apoptosis was investigated by cell cycle analysis, terminal deoxytransferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay, and staining with monoclonal antibody APO2.7 reacting with 7A6 antigen. The percentage of the hypodiploid fraction by cell cycle analysis and the percentage of apoptosis determined by TUNEL assay were significantly higher in HHV-6-infected CBMC compared with uninfected CBMC. 7A6 antigen, induced on the mitochondria membrane in apoptotic cells, were mainly expressed in CD4+ cells. 7A6 antigen was also detected in HHV-6-infected cells determined by monoclonal antibody OHV-3 reacting with HHV-6 glycoprotein. These data indicated that HHV-6 induced apoptosis in HHV-6-infected cells after stimulation with IL-2 for 5 days. The addition of anti-Fas antibody, anti-Fas ligand antibody, and anti-TNF-alpha antibody did not affect the induction of apoptosis by HHV-6, indicating that the Fas-Fas ligand pathway and TNF pathway did not contribute to the apoptosis induced by HHV-6.
研究了人类疱疹病毒6型（HHV - 6）诱导脐血淋巴细胞凋亡的情况。用植物血凝素（PHA）预刺激的脐血单个核细胞（CBMC）感染HHV - 6，并与白细胞介素2（IL - 2）一起培养5天。通过细胞周期分析、末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）测定法以及用与7A6抗原反应的单克隆抗体APO2.7染色来研究细胞凋亡。与未感染的CBMC相比，通过细胞周期分析得出的亚二倍体部分百分比以及通过TUNEL测定法确定的细胞凋亡百分比在HHV - 6感染的CBMC中显著更高。凋亡细胞线粒体膜上诱导产生的7A6抗原主要在CD4 +细胞中表达。通过与HHV - 6糖蛋白反应的单克隆抗体OHV - 3在HHV - 6感染的细胞中也检测到了7A6抗原。这些数据表明，HHV - 6在经IL - 2刺激5天后在HHV - 6感染的细胞中诱导了细胞凋亡。添加抗Fas抗体、抗Fas配体抗体和抗TNF -α抗体并不影响HHV - 6诱导的细胞凋亡，这表明Fas - Fas配体途径和TNF途径与HHV - 6诱导的细胞凋亡无关。
