Yasukawa M, Inoue Y, Ohminami H, Terada K, Fujita S
First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Japan.
J Gen Virol. 1998 Jan;79 ( Pt 1):143-7. doi: 10.1099/0022-1317-79-1-143.
The present authors have recently reported that inoculation with human herpesvirus-6 (HHV-6) renders CD4+ T lymphocytes susceptible to apoptosis in vitro. In order to confirm that apoptosis of CD4+ T lymphocytes also occurs in HHV-6 infection in vivo, apoptosis of lymphocytes isolated from nine patients with exanthem subitum and from an adult patient with severe HHV-6 infection was examined. Peripheral blood mononuclear cells were cultured for 3 days and apoptosis of lymphocytes was then examined by flow cytometry of propidium iodide-stained DNA. The percentages of hypodiploid DNA, indicating apoptosis, in lymphocytes from 10 patients with HHV-6 infection were significantly higher than those from five infant patients with noninfectious diseases and five healthy adults (P < 0-0002). DNA fragmentation was also detected by agarose gel electrophoresis in lymphocytes from patients with HHV-6 infection. Apoptosis appeared to occur predominantly in CD4+ T lymphocytes and HHV-6 was isolated from the CD4+ T lymphocyte fraction. These data demonstrate that HHV-6 renders CD4+ T lymphocytes susceptible to apoptosis in vivo.
本文作者最近报道，接种人疱疹病毒6型（HHV - 6）可使CD4 + T淋巴细胞在体外易于发生凋亡。为了证实CD4 + T淋巴细胞的凋亡在体内HHV - 6感染中也会发生，对9例幼儿急疹患者和1例严重HHV - 6感染成年患者分离出的淋巴细胞的凋亡情况进行了检测。外周血单个核细胞培养3天，然后通过碘化丙啶染色DNA的流式细胞术检测淋巴细胞的凋亡情况。10例HHV - 6感染患者淋巴细胞中显示凋亡的亚二倍体DNA百分比显著高于5例非感染性疾病婴儿患者和5例健康成年人（P < 0.0002）。通过琼脂糖凝胶电泳也在HHV - 6感染患者的淋巴细胞中检测到了DNA片段化。凋亡似乎主要发生在CD4 + T淋巴细胞中，并且从CD4 + T淋巴细胞组分中分离出了HHV - 6。这些数据表明，HHV - 6可使CD4 + T淋巴细胞在体内易于发生凋亡。
