一种新的哺乳动物TATA结合蛋白相关因子的转录功能

Transcriptional functions of a new mammalian TATA-binding protein-related factor.

作者信息

Maldonado E

机构信息

Programa de Biologia Celular y Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiago 7, Chile.

出版信息

J Biol Chem. 1999 May 7;274(19):12963-6. doi: 10.1074/jbc.274.19.12963.

DOI:10.1074/jbc.274.19.12963

PMID:10224042

Abstract

A mammalian protein highly homologous to TATA-binding protein (TBP) has been identified and cloned. The recombinant mammalian TBP-related factor binds to the TATA box of the Ad-MLP and forms stable complexes with TFIIB on the promoter DNA. The mammalian TBP-related factor is able to substitute for TBP in supporting transcription by RNA polymerase II in an in vitro reconstituted system.

摘要

一种与TATA结合蛋白（TBP）高度同源的哺乳动物蛋白已被鉴定和克隆。重组的哺乳动物TBP相关因子与腺病毒主要晚期启动子（Ad-MLP）的TATA盒结合，并在启动子DNA上与TFIIB形成稳定复合物。在体外重组系统中，该哺乳动物TBP相关因子能够替代TBP来支持RNA聚合酶II的转录。