在手性含麦角灵骨架固定相上直接拆分旋光异构体。6. 芳基丙酸类药物的对映体拆分。

Direct resolution of optically active isomers on chiral packings containing ergoline skeleton. 6. Enantioseparation of profens.

作者信息

Olsovská J, Flieger M, Bachechi F, Messina A, Sinibaldi M

机构信息

Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

出版信息

Chirality. 1999;11(4):291-300. doi: 10.1002/(SICI)1520-636X(1999)11:4<291::AID-CHIR6>3.0.CO;2-4.

DOI:10.1002/(SICI)1520-636X(1999)11:4<291::AID-CHIR6>3.0.CO;2-4

PMID:10224656

Abstract

The enantioselective behaviour of some underivatized 2-arylpropionic acids (profens) and flobufen by HPLC using a terguride-based chiral stationary phase was tested. X-ray analysis of crystals of the chiral selector and its complexes with naproxen allowed a deeper insight into the enantiodiscriminative process. The column stability and reproducibility, and the potential of the packing for semipreparative scale separations were also determined. A method for determining flobufen enantiomers and metabolites in plasma samples is described.

摘要

使用基于特古利德的手性固定相，通过高效液相色谱法（HPLC）测试了一些未衍生化的2-芳基丙酸（洛芬类药物）和氟比洛芬的对映选择性行为。对手性选择剂及其与萘普生的配合物的晶体进行X射线分析，有助于更深入地了解对映体鉴别过程。还测定了色谱柱的稳定性和重现性，以及该填料用于半制备规模分离的潜力。描述了一种测定血浆样品中氟比洛芬对映体和代谢物的方法。