Sinibaldi M, Vinci M, Federici F, Flieger M
Istituto di Cromatografia, CNR-Area della Ricerca di Roma, Italy.
Biomed Chromatogr. 1997 Sep-Oct;11(5):307-10. doi: 10.1002/(SICI)1099-0801(199709)11:5<307::AID-BMC704>3.0.CO;2-T.
Enantioseparation of 2-arylpropionic acids (Flobufen) and dansyl-derivatives of amino acids can be achieved in 10-15 min by electrochromatography using a capillary coated with poly-terguride. It was found that the analytes, in the range of the buffer pH between 2.5-4.0, were driven by anodic electroosmotic flow originated by the positively charged moieties of the ergolinic skeleton, and, only partially, by their anodic electrophoretic mobility. The enantiomers eluted with the same sequence, e.g. L- or (S)-isomer more retained than D- or (R)-, which was observed in HPLC separations on terguride-silica based CSPs, thus indicating that racemates were resolved by a similar chiral recognition mechanism. The retention of the solutes was affected by the concentration and composition of the effluent. An other mobile phase variable, strongly affecting the selectivity, was the pH of the buffer.
使用涂有聚麦角灵的毛细管通过电色谱法可在10 - 15分钟内实现2 - 芳基丙酸（氟比洛芬）和氨基酸的丹磺酰衍生物的对映体分离。研究发现，在缓冲液pH值为2.5 - 4.0的范围内，分析物由麦角灵骨架带正电荷部分产生的阳极电渗流驱动，且仅部分由其阳极电泳迁移率驱动。对映体以相同顺序洗脱，例如L - 或（S） - 异构体比D - 或（R） - 异构体保留时间更长，这与基于麦角灵 - 硅胶的手性固定相上的高效液相色谱分离情况相同，因此表明外消旋体是通过类似的手性识别机制拆分的。溶质的保留受流出物浓度和组成的影响。另一个强烈影响选择性的流动相变量是缓冲液的pH值。
