Menu E, Reynes J M, Müller-Trutwin M C, Guillemot L, Versmisse P, Chiron M, An S, Trouplin V, Charneau P, Fleury H, Barré-Sinoussi F, Sainte Marie F F
Institut Pasteur, Unité de Biologie des Rétrovirus, Paris, France.
J Acquir Immune Defic Syndr Hum Retrovirol. 1999 Apr 15;20(5):481-7. doi: 10.1097/00042560-199904150-00011.
To investigate the genetic and biologic features of HIV-1 strains circulating in Cambodia, viruses from 95 HIV-1-seropositive individuals were subtyped by heteroduplex mobility assay (HMA) and 23 were further analyzed for their biologic characteristics. Eighty-nine individuals were clearly infected by HIV-1 subtype E. The other six samples were sequenced, together with 17 HMA subtype E samples. All but one of the 23 Cambodian env sequences clustered with previously described Thai and Vietnamese subtype E sequences, bearing a GPGQ motif at the tip of the V3 loop; the last had a GPGR motif and was phylogenetically equidistant from Asian and African subtype E viruses. Nonsyncytium-inducing, CCR5-dependent viruses predominated in patients of clinical stage B even in some with a high viral load and were detected in about 50% of the patients of stage C. All syncytium-inducing strains, mostly from AIDS patients, used both CCR5 and CXCR4. The presence of syncytium-inducing viruses did not correlate with the plasma viral load. These data show that CCR5-dependent HIV-1 subtype E is currently predominant in Cambodia. The analysis of clinical and virologic markers strongly supports the idea that dynamics of the viral population during subtype E infection in Southeast Asia is similar to that of subtype B infection in Europe and the United States.
为了研究柬埔寨流行的HIV-1毒株的基因和生物学特征，采用异源双链迁移分析（HMA）对95例HIV-1血清阳性个体的病毒进行亚型分析，并对其中23株病毒的生物学特性进行了进一步分析。89例个体明确感染了HIV-1 E亚型。对另外6个样本以及17个HMA E亚型样本进行了测序。23个柬埔寨env序列中，除1个外，其余均与先前描述的泰国和越南E亚型序列聚类，在V3环顶端带有GPGQ基序；最后1个具有GPGR基序，在系统发育上与亚洲和非洲E亚型病毒距离相等。非合胞体诱导的、依赖CCR5的病毒在临床B期患者中占主导地位，即使在一些病毒载量高的患者中也是如此，并且在约50%的C期患者中被检测到。所有合胞体诱导株，大多来自艾滋病患者，同时使用CCR5和CXCR4。合胞体诱导病毒的存在与血浆病毒载量无关。这些数据表明，依赖CCR5的HIV-1 E亚型目前在柬埔寨占主导地位。对临床和病毒学标志物的分析有力地支持了这样一种观点，即东南亚E亚型感染期间病毒群体的动态与欧美B亚型感染相似。
