Pool E J, Bouic P
Department of Medical Microbiology, University of Stellenbosch, Tygerberg, South Africa.
J Immunoassay. 1999 Feb-May;20(1-2):1-11. doi: 10.1080/01971529909349310.
The aim of this study was to investigate whether the ex vivo whole blood culture (WBC) assay system can be used to detect pyrogens in blood from patients with symptoms of sepsis. Blood samples from 35 patients with symptoms of sepsis were assayed for bacterial contamination using the radiometric blood culture assay. Serum from the same patients were screened for IL-6, C-reactive protein (CRP) and pyrogens using the whole blood culture assay. Serum samples from 26 patients tested positive for pyrogens. Of the 26 patients with pyrogenic serum, 15 had elevated serum IL-6 levels and 19 had elevated CRP levels. Only two of the samples had positive blood cultures as detected by the routine radiometric assay. Both of these patients had high serum CRP and pyrogen levels, while only one of them had an elevated serum IL-6 level. These results show that the WBC is very sensitive in detecting pyrogens in serum of patients. This technique can be a useful tool to quantitate pyrogens in sera from patients with symptoms of sepsis and to determine whether their clinical symptoms are caused by pyretic substances in their circulatory system.
本研究的目的是调查体外全血培养（WBC）检测系统是否可用于检测有败血症症状患者血液中的热原。使用放射性血液培养检测法对35例有败血症症状患者的血样进行细菌污染检测。使用全血培养检测法对同一批患者的血清进行白细胞介素-6（IL-6）、C反应蛋白（CRP）和热原筛查。26例患者的血清样本热原检测呈阳性。在这26例血清有热原的患者中，15例血清IL-6水平升高，19例CRP水平升高。通过常规放射性检测法，仅两份样本血培养呈阳性。这两名患者血清CRP和热原水平均较高，但其中只有一人血清IL-6水平升高。这些结果表明，全血培养在检测患者血清中的热原方面非常敏感。该技术可作为一种有用的工具，用于定量有败血症症状患者血清中的热原，并确定其临床症状是否由循环系统中的致热物质引起。
