Cahill C M, Rogers J T
Dept. of Medicine, Massachusetts General Hospital, Boston 02114, USA.
J Immunoassay. 1999 Feb-May;20(1-2):45-55. doi: 10.1080/01971529909349313.
Antisera were raised in rabbits against two mouse monoclonal anti-porcine growth hormone (pGH) antibodies. Anti-idiotypic antibodies were isolated from rabbit sera by successive passage over immunoadsorbent columns of normal mouse Ig (mIg), followed by the specific immunizing monoclonal and elution from the latter. Enzyme linked immunoadsorbent assay (ELISA) for anti-idiotype and free idiotype were established. While showing specificity for their respective inducing monoclonals, anti-idiotypes also cross reacted in varying degrees with other anti-pGH monoclonals regardless of specificity differences between the antibodies, demonstrating the presence of a cross-reactive idiotype.
用两种小鼠抗猪生长激素（pGH）单克隆抗体在兔体内制备抗血清。通过在正常小鼠Ig（mIg）免疫吸附柱上连续传代，然后用特异性免疫单克隆抗体处理并从后者洗脱，从兔血清中分离出抗独特型抗体。建立了抗独特型和游离独特型的酶联免疫吸附测定（ELISA）。抗独特型抗体虽然对各自诱导的单克隆抗体具有特异性，但也与其他抗pGH单克隆抗体有不同程度的交叉反应，而不管抗体之间的特异性差异如何，这表明存在交叉反应独特型。
