Suppr超能文献

抗单克隆抗孕酮抗体的抗独特型血清：在兔和大鼠中的制备及特异性鉴定

Anti-idiotypic sera against monoclonal anti-progesterone antibodies: production in rabbits and rats and characterization of specificity.

作者信息

Taussig M J, Brown N, Ellis S, Holliman A, Peat D, Richardson N, Heap R B, Feinstein A

出版信息

Immunology. 1986 Jul;58(3):445-52.

PMID:3488262
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1453462/
Abstract

Antisera were raised in rabbits and rats against three mouse monoclonal anti-progesterone IgG1 antibodies. Anti-idiotypic antibodies were isolated from rabbit sera by successive passage over immunoadsorbent columns of normal mouse Ig and the specific immunizing monoclonal, with elution from the latter. Radioimmunoassays for anti-idiotype and free idiotype were established, enabling detection of idiotype in sera of mice immunized with progesterone-BSA conjugate. Binding of rabbit anti-idiotype to anti-progesterone monoclonals was partially inhibitable by free progesterone-hemisuccinate or progesterone-ovalbumin conjugate. While showing considerable specificity for their respective inducing monoclonals, the anti-idiotypes also cross-reacted in varying degrees with the other anti-progesterone monoclonals, demonstrating the presence of IdI and IdX determinants. The patterns of cross-reactivity showed some correlation with the relative isoelectric points and combining-site specificities of the anti-progesterones. The specificities of rat and rabbit anti-idiotypes were similar, but not identical.

摘要

用兔和大鼠制备了针对三种小鼠抗孕酮IgG1单克隆抗体的抗血清。通过在正常小鼠Ig和特异性免疫单克隆抗体的免疫吸附柱上连续传代，从兔血清中分离出抗独特型抗体，并从后者进行洗脱。建立了抗独特型和游离独特型的放射免疫测定法，能够检测用孕酮-BSA缀合物免疫的小鼠血清中的独特型。游离孕酮半琥珀酸酯或孕酮-卵清蛋白缀合物可部分抑制兔抗独特型与抗孕酮单克隆抗体的结合。虽然抗独特型抗体对各自诱导的单克隆抗体表现出相当高的特异性，但它们也与其他抗孕酮单克隆抗体有不同程度的交叉反应，表明存在IdI和IdX决定簇。交叉反应模式与抗孕酮抗体的相对等电点和结合位点特异性有一定相关性。大鼠和兔抗独特型抗体的特异性相似，但不完全相同。

相似文献

1
Anti-idiotypic sera against monoclonal anti-progesterone antibodies: production in rabbits and rats and characterization of specificity.抗单克隆抗孕酮抗体的抗独特型血清：在兔和大鼠中的制备及特异性鉴定
Immunology. 1986 Jul;58(3):445-52.
2
Anti-idiotypic sera against monoclonal anti-porcine growth hormone antibodies: production in rabbits and characterization of specificity.抗单克隆抗猪生长激素抗体的抗独特型血清：在兔体内的产生及特异性鉴定
J Immunoassay. 1999 Feb-May;20(1-2):45-55. doi: 10.1080/01971529909349313.
3
Analysis of the anti-alpha 1 leads to 3 dextran response with monoclonal anti-idiotype antibodies.用单克隆抗独特型抗体分析抗α1导致3葡聚糖反应。
J Immunol. 1983 Sep;131(3):1375-9.
4
Mapping the idiotopes of a monoclonal anti-myoglobin antibody with syngeneic monoclonal anti-idiotypic antibodies: detection of a common idiotope.用同基因单克隆抗独特型抗体定位一种单克隆抗肌红蛋白抗体的独特型表位：共同独特型表位的检测
J Immunol. 1986 Feb 1;136(3):1007-14.
5
Anti-idiotypic antibody used for the localization of parenterally administered monoclonal anti-progesterone antibody in mice.用于在小鼠中定位经肠胃外给药的单克隆抗孕酮抗体的抗独特型抗体。
Scand J Immunol. 1988 Sep;28(3):367-76. doi: 10.1111/j.1365-3083.1988.tb01462.x.
6
Idiotype-anti-idiotype interactions of VHIX-coded anti-progesterone and anti-arsonate antibodies. Comparison of passive haemagglutination and radioimmunoassays.VHIX编码的抗孕酮和抗砷酸盐抗体的独特型-抗独特型相互作用。被动血凝试验和放射免疫测定的比较。
Scand J Immunol. 1987 Sep;26(3):267-76. doi: 10.1111/j.1365-3083.1987.tb02260.x.
7
A monoclonal anti-idiotypic antibody which mimics angiotensin II in inducing a population of anti-hormone antibodies.一种单克隆抗独特型抗体，在诱导一群抗激素抗体方面模拟血管紧张素II。
Hybridoma. 1987 Oct;6(5):461-8. doi: 10.1089/hyb.1987.6.461.
8
Anti-idiotypes against anti-H-2 antibodies VI. Detection of shared idiotypes among monoclonal anti-H-2 antibodies.抗抗H-2抗体的独特型抗体VI. 单克隆抗H-2抗体间共享独特型的检测
Eur J Immunol. 1983 Jun;13(6):489-95. doi: 10.1002/eji.1830130611.
9
Anti-idiotypic antisera raised against monoclonal antibody specific for a p24 gag region epitope detects a common interspecies idiotype associated with anti-HIV responses.针对p24 gag区域表位的单克隆抗体产生的抗独特型抗血清，可检测到一种与抗HIV反应相关的常见种间独特型。
Viral Immunol. 1990 Summer;3(2):99-109. doi: 10.1089/vim.1990.3.99.
10
Blocking of pregnancy in mice by immunization with anti-idiotype directed against monoclonal anti-progesterone antibody.用针对单克隆抗孕酮抗体的抗独特型抗体免疫小鼠来阻断妊娠
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7098-102. doi: 10.1073/pnas.86.18.7098.

引用本文的文献

1
Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.D-JH连接区突变对抗孕酮抗体DB3亲和力、特异性及独特型的影响
Protein Sci. 2006 Sep;15(9):2141-8. doi: 10.1110/ps.062236806. Epub 2006 Aug 1.
2
Anti-anti-idiotypic (Ab3) antibodies that bind progesterone-11alpha-bovine serum albumin differ in their combining sites from antibodies raised directly against the antigen.与孕酮-11α-牛血清白蛋白结合的抗抗独特型（Ab3）抗体，其结合位点与直接针对该抗原产生的抗体不同。
Immunology. 2000 Jun;100(2):152-64. doi: 10.1046/j.1365-2567.2000.00006.x.
3
Characterization of a progesterone-binding, three-domain antibody fragment (VH/K) expressed in Escherichia coli.在大肠杆菌中表达的一种孕酮结合三结构域抗体片段（VH/K）的特性分析。
Immunology. 1995 Apr;84(4):662-8.
4
Blocking of pregnancy in mice by immunization with anti-idiotype directed against monoclonal anti-progesterone antibody.用针对单克隆抗孕酮抗体的抗独特型抗体免疫小鼠来阻断妊娠
Proc Natl Acad Sci U S A. 1989 Sep;86(18):7098-102. doi: 10.1073/pnas.86.18.7098.
5
Anti-idiotypes used in immunohistochemistry.免疫组织化学中使用的抗独特型抗体。
Histochem J. 1990 Oct;22(10):519-25. doi: 10.1007/BF01005973.
6
Induction of anti-progesterone immunity and pregnancy blocking by anti-progesterone anti-idiotypes. Variable efficacy of polyclonal Ab2 antibodies directed against a panel of closely related Ab1 antibodies.抗孕酮抗独特型抗体诱导抗孕酮免疫及阻断妊娠。针对一组密切相关的Ab1抗体的多克隆Ab2抗体的不同效力。
Immunology. 1991 Jul;73(3):348-55.
7
Blot-sequencing of antibodies: application to analysis of V gene usage among anti-steroid monoclonal antibodies.抗体的印迹测序：在抗类固醇单克隆抗体中V基因使用情况分析的应用
Immunology. 1991 Apr;72(4):471-80.

本文引用的文献

1
Individual Antigenic Specificity of Isolated Antibodies.分离抗体的个体抗原特异性
Science. 1963 Jun 14;140(3572):1218-9. doi: 10.1126/science.140.3572.1218.
2
Progesterone monoclonal antibody blocks pregnancy in mice.孕酮单克隆抗体可阻止小鼠怀孕。
Nature. 1982 Feb 4;295(5848):415-7. doi: 10.1038/295415a0.
3
The kinetics of antibody binding to membrane antigens in solution and at the cell surface.抗体与溶液中和细胞表面膜抗原结合的动力学。
Biochem J. 1980 Apr 1;187(1):1-20. doi: 10.1042/bj1870001.
4
Antibody response to dinitrophenyl hapten by foetal, neonatal and young pigs.胎儿、新生仔猪和幼猪对二硝基苯基半抗原的抗体反应。
Immunology. 1983 Apr;48(4):703-11.
5
Action of anti-progesterone monoclonal antibody in blocking pregnancy after postcoital administration in mice.抗孕酮单克隆抗体在小鼠交配后给药阻断妊娠中的作用。
J Endocrinol. 1984 Apr;101(1):95-100. doi: 10.1677/joe.0.1010095.
6
Immunoglobulin structure--function correlates: antigen binding and idiotypes.免疫球蛋白结构与功能的相关性：抗原结合与独特型
Contemp Top Mol Immunol. 1983;9:169-209. doi: 10.1007/978-1-4684-4517-6_6.
7
Anti-idiotype and anti-hormone receptor antibodies.抗独特型抗体和抗激素受体抗体。
Springer Semin Immunopathol. 1983;6(1):67-78. doi: 10.1007/BF01857367.
8
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
9
Passive immunization against progesterone inhibits endometrial sensitization in pseudopregnant mice and has antifertility effects in pregnant mice which are reversible by steroid treatment.被动免疫黄体酮可抑制假孕小鼠的子宫内膜致敏，并对妊娠小鼠产生抗生育作用，而这种作用可通过类固醇治疗逆转。
J Endocrinol. 1985 Jan;104(1):153-8. doi: 10.1677/joe.0.1040153.
10
Multiple individual and cross-specific indiotypes on 13 levan-binding myeloma proteins of BALB/c mice.BALB/c小鼠13种结合果聚糖的骨髓瘤蛋白上的多种个体型和交叉特异性个体型。
J Exp Med. 1975 Jul 1;142(1):106-19. doi: 10.1084/jem.142.1.106.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验