Bakhiet M, Ozenci V, Withagen C, Mustafa M, Fredrikson S, Link H
Divisions of Infectious Diseases and Neurology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden.
Clin Diagn Lab Immunol. 1999 May;6(3):415-9. doi: 10.1128/CDLI.6.3.415-419.1999.
Multiple sclerosis (MS) is a demyelinating disorder of the central nervous system of unknown etiology. Immune mechanisms involving the proinflammatory cytokine gamma interferon (IFN-gamma) are believed to play an important role in the pathogenesis of MS. IFN-beta-1b has been introduced as a treatment for MS and was found to reduce the number and severity of clinical exacerbations. To examine the influence of IFN-beta-1b on myelin basic protein (MBP)-specific and phytohemagglutinin-induced IFN-gamma production, we developed a cell-released capturing enzyme-linked immunosorbent assay (CRC-ELISA), which rapidly measures spontaneous and antigen- or mitogen-induced cellular IFN-gamma production. CRC-ELISA documented a significant MBP-specific T-cell response in the blood of untreated MS patients, as assessed by IFN-gamma production. This response was suppressed in MS patients treated with IFN-beta-1b. The present work confirms in vivo the in vitro suppressive effects of IFN-beta-1b on IFN-gamma production in MS. Moreover, it provides a powerful new technique for detection of cytokines.
多发性硬化症（MS）是一种病因不明的中枢神经系统脱髓鞘疾病。涉及促炎细胞因子γ干扰素（IFN-γ）的免疫机制被认为在MS的发病机制中起重要作用。IFN-β-1b已被引入作为MS的一种治疗方法，并且发现它能减少临床病情加重的次数和严重程度。为了研究IFN-β-1b对髓鞘碱性蛋白（MBP）特异性和植物血凝素诱导的IFN-γ产生的影响，我们开发了一种细胞释放捕获酶联免疫吸附测定法（CRC-ELISA），该方法可快速测量自发的以及抗原或丝裂原诱导的细胞IFN-γ产生。通过IFN-γ产生评估，CRC-ELISA记录了未经治疗的MS患者血液中显著的MBP特异性T细胞反应。在用IFN-β-1b治疗的MS患者中，这种反应受到抑制。目前的研究在体内证实了IFN-β-1b对MS中IFN-γ产生的体外抑制作用。此外，它为细胞因子的检测提供了一种强大的新技术。
