抗白色念珠菌天冬氨酸蛋白酶单克隆抗体的制备、鉴定及表位作图
Production, characterization, and epitope mapping of a monoclonal antibody against aspartic proteinase of Candida albicans.
作者信息
Na B K, Chung G T, Song C Y
机构信息
Department of Biology and Institute of Basic Science, College of Natural Science, Chung-Ang University, Seoul 156-756, Korea.
出版信息
Clin Diagn Lab Immunol. 1999 May;6(3):429-33. doi: 10.1128/CDLI.6.3.429-433.1999.
A monoclonal antibody (MAb; MAb CAP1) that was reactive with extracellular aspartic proteinase of Candida albicans (CAP) was produced. The MAb showed strong sensitivity and reactivity to CAP but not to the aspartic proteinases of Candida parapsilosis, Candida tropicalis, and Aspergillus fumigatus or to human cathepsin D or porcine pepsin. The epitope of the CAP recognized by the MAb was the proteinaseous part of CAP and the putative epitope of the MAb was located in the Asp77 to Gly103 sequence. This antibody could be useful for the characterization of CAP and would be a valuable probe for the detection of CAP antigen in the sera of patients with invasive candidiasis.
制备了一种与白色念珠菌胞外天冬氨酸蛋白酶（CAP）反应的单克隆抗体（MAb；MAb CAP1）。该单克隆抗体对CAP表现出强烈的敏感性和反应性，但对近平滑念珠菌、热带念珠菌和烟曲霉的天冬氨酸蛋白酶、人组织蛋白酶D或猪胃蛋白酶无反应。该单克隆抗体识别的CAP表位是CAP的蛋白质部分，且该单克隆抗体的推定表位位于Asp77至Gly103序列中。这种抗体可用于CAP的特性鉴定，并且将是检测侵袭性念珠菌病患者血清中CAP抗原的有价值探针。
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