Satoh K, Kihara T, Ida Y, Sakagami H, Koyama N, Premanathan M, Arakaki R, Nakashima H, Komatsu N, Fujimaki M, Misawa Y, Hata N
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Anticancer Res. 1999 Jan-Feb;19(1A):357-64.
The radical modulation activity of lignins prepared from the cone of Pinus elliottii var. Elliottii was investigated, using ESR spectroscopy. These lignins produced radical(s) under alkaline conditions, and the radical intensity was increased with increasing pH. Lower concentrations of lignins slightly reduced the radical intensity of sodium ascorbate, whereas higher concentrations of lignins enhanced both the radical intensity and cytotoxic activity of sodium ascorbate. Lignins effectively scavenged superoxide anion, produced by hypoxanthine-xanthine oxidase reaction. Elliottii lignins significantly inhibited the human immunodeficiency virus (HIV)-induced cytopathic effect, in similar fashions to other natural, commercial and synthetic lignins. Pretreatment of mice with lignins significantly protected them from the lethal infection with E. coli. Crude alkaline extracts of Elliottii pine cone displayed similar magnitude of activity with lignins. These data further supports the medicinal efficacy of plant extracts.
利用电子自旋共振光谱法研究了湿地松球果制备的木质素的自由基调制活性。这些木质素在碱性条件下产生自由基，且自由基强度随pH值升高而增加。较低浓度的木质素会轻微降低抗坏血酸钠的自由基强度，而较高浓度的木质素则会增强抗坏血酸钠的自由基强度和细胞毒性活性。木质素能有效清除次黄嘌呤 - 黄嘌呤氧化酶反应产生的超氧阴离子。湿地松木质素以与其他天然、商业和合成木质素相似的方式显著抑制人类免疫缺陷病毒（HIV）诱导的细胞病变效应。用木质素预处理小鼠可显著保护它们免受大肠杆菌致死性感染。湿地松松果的粗碱性提取物显示出与木质素相似程度的活性。这些数据进一步支持了植物提取物的药用功效。
