Satoh K, Anzai S, Sakagami H
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Anticancer Res. 1998 Mar-Apr;18(2A):833-7.
The effect of hot water and alkaline extracts of Acer nikoense Maxim. on the radical intensity of sodium ascorbate, superoxide anion (O2-)(produced by hypoxanthine-xanthine oxidase reaction) and hydroxyl radical (-OH)(generated by Fenton reaction) was investigated using ESR spectroscopy. All these extracts produced radical(s) under alkaline conditions and enhanced the radical intensity of sodium ascorbate. Among these fractions, Fr. I showed the highest stimulation effect, which coincided with its higher degradation rate and capacity to enhance the cytotoxic activity of ascorbate. These fractions also dose-dependently reduced the radical intensity of O2- and -OH. The present study demonstrates that Acer nikoense Maxim. extracts potentiate both radical intensity and apoptosis-inducing activity of ascorbate.
日本槭热水提取物和碱性提取物对抗坏血酸钠、超氧阴离子（由次黄嘌呤-黄嘌呤氧化酶反应产生）和羟自由基（由芬顿反应产生）的自由基强度的影响，采用电子自旋共振光谱法进行了研究。所有这些提取物在碱性条件下均产生自由基，并增强了抗坏血酸钠的自由基强度。在这些组分中，组分I表现出最高的刺激作用，这与其较高的降解率和增强抗坏血酸细胞毒性活性的能力相一致。这些组分还呈剂量依赖性地降低了超氧阴离子和羟自由基的自由基强度。本研究表明，日本槭提取物增强了抗坏血酸的自由基强度和诱导凋亡活性。
