Hänze J, Berthold A, Klammt J, Gallaher B, Siebler T, Kratzsch J, Elmlinger M, Kiess W
Children's Hospital, University of Giessen, Germany.
Horm Metab Res. 1999 Feb-Mar;31(2-3):77-9. doi: 10.1055/s-2007-978702.
The insulin receptor related receptor (IRR) is a heterotetrameric transmembrane receptor with intrinsic tyrosine kinase activity. The IRR shares large homology with the insulin and the insulin-like growth factor-1 (IGF-I) receptor with regard to amino acid sequence and protein structure. So far, only a partial human sequence containing the complete 3' end has been reported, although the full-length human IRR cDNA had been used for transfection studies and functional analysis of the receptor. We have isolated a full-length human IRR cDNA and report on the 5' translated and untranslated region of the human IRR gene. The full length IRR sequence contains 4150 bases and shares a high degree of homology with the guinea pig IRR cDNA sequence and rat IRR sequences that had been reported earlier on by others. Sequencing of the IRR cDNA revealed that the human IRR cDNA contains 341 bases corresponding to the IRR 5' end in addition to the bases that had been reported on before. Also, this sequence contains the start codon of translation. The full length cDNA for the human IRR can now be used for functional expression studies and to elucidate the nature of the ligand for this receptor type.
胰岛素受体相关受体（IRR）是一种具有内在酪氨酸激酶活性的异源四聚体跨膜受体。在氨基酸序列和蛋白质结构方面，IRR与胰岛素及胰岛素样生长因子-1（IGF-I）受体具有高度同源性。尽管全长人类IRR cDNA已用于该受体的转染研究和功能分析，但迄今为止，仅报道了包含完整3'端的部分人类序列。我们分离出了全长人类IRR cDNA，并报告了人类IRR基因的5'翻译区和非翻译区。全长IRR序列包含4150个碱基，与豚鼠IRR cDNA序列以及他人先前报道的大鼠IRR序列具有高度同源性。IRR cDNA测序显示，除了之前报道的碱基外，人类IRR cDNA还包含对应于IRR 5'端的341个碱基。此外，该序列包含翻译起始密码子。人类IRR的全长cDNA现在可用于功能表达研究，并阐明这种受体类型的配体性质。
