Shier P, Watt V M
Department of Physiology, University of Toronto, Ontario, Canada.
Mol Endocrinol. 1992 May;6(5):723-9. doi: 10.1210/mend.6.5.1603082.
Characterization of genomic DNA encoding the insulin receptor-related receptor (IRR) previously revealed that the predicted IRR protein is closely related to the insulin and insulin-like growth factor-I receptor protein-tyrosine kinases. Using rat IRR genomic DNA as probe, IRR transcripts were detected by Northern blot analysis in RNA from rat kidney, stomach, and thymus, but not in RNA from other tissues, including skeletal muscle, brain, intestine, and uterus. Primer extension analysis using RNA from stomach revealed a single transcriptional start site 29 basepairs down-stream from a putative TATA box and 544 basepairs up-stream of the initiator methionine codon. Amplification of IRR cDNA by polymerase chain reaction and isolation of partial IRR cDNA clones confirmed that the IRR gene is an expressed gene.
对编码胰岛素受体相关受体（IRR）的基因组DNA的特性分析先前已揭示，预测的IRR蛋白与胰岛素及胰岛素样生长因子-I受体蛋白酪氨酸激酶密切相关。以大鼠IRR基因组DNA为探针，通过Northern印迹分析在大鼠肾脏、胃和胸腺的RNA中检测到了IRR转录本，但在包括骨骼肌、脑、肠和子宫在内的其他组织的RNA中未检测到。使用胃组织的RNA进行引物延伸分析，结果显示在假定的TATA框下游29个碱基对以及起始甲硫氨酸密码子上游544个碱基对处有一个单一的转录起始位点。通过聚合酶链反应扩增IRR cDNA并分离部分IRR cDNA克隆，证实IRR基因是一个表达基因。
