Maitz P K, Lanzetta M, Owen E R
Microsearch Foundation of Australia, Sydney.
J Reconstr Microsurg. 1999 Apr;15(3):177-81. doi: 10.1055/s-2007-1000089.
In this study, a unilateral epigastric free flap was raised in 12 rabbits. After the arterial portion of the flap (the superficial femoral artery) was replaced by a 1-cm-long polytetrafluoroethylene graft with an internal diameter of 1 mm, the flap was revascularized by two conventional microvascular end-to-end anastomoses (graft to artery and vein to vein). At 3 weeks, all flaps were raised again and the patency of the polytetrafluoroethylene grafts was checked. All grafts, including the proximal and distal anastomoses, were harvested and processed for light and electron microscopy. All grafts remained patient, and all flaps survived the period of 3 weeks. At reexploration, the graft was completely covered with connective tissue. Light and scanning electron microscopy evaluation showed that the internal surface of the graft was covered by a fibrin layer, and that the ingrowth of neoendothelium had just started from both anastomotic sites. The use of small-diameter polytetrafluoroethylene grafts in a rabbit free-flap model was demonstrated to be a reliable alternative for pedicle elongation.
在本研究中，对12只兔子掀起单侧腹壁游离皮瓣。在用内径1毫米、长度1厘米的聚四氟乙烯移植物替换皮瓣的动脉部分（股浅动脉）后，通过两次传统的微血管端端吻合术（移植物与动脉、静脉与静脉）使皮瓣重新血管化。3周时，再次掀起所有皮瓣并检查聚四氟乙烯移植物的通畅情况。采集所有移植物，包括近端和远端吻合口，进行光镜和电镜检查。所有移植物均保持通畅，所有皮瓣均存活3周。再次探查时，移植物完全被结缔组织覆盖。光镜和扫描电镜评估显示，移植物内表面覆盖有一层纤维蛋白层，新内皮细胞从两个吻合口处刚刚开始向内生长。在兔游离皮瓣模型中使用小直径聚四氟乙烯移植物被证明是延长蒂部的可靠替代方法。
