Steinfeld S, Penaloza A, Ribaï P, Decaestecker C, Danguy A, Gabius H J, Salmon I, Appelboom T, Kiss R
Department of Rheumatology, Erasmus Academic Hospital, Brussels, Belgium.
J Rheumatol. 1999 Apr;26(4):833-41.
Sjögren's syndrome (SS) is an autoimmune exocrinopathy. The mannose binding lectin (MBL), a pluripotent molecule of the innate immune system, is involved in the pathogenesis of autoimmune diseases. We investigated whether specific ligands for MBL and MBL related structures could be reliable markers in cases of SS.
The labial salivary glands of 19 patients fulfilling the diagnostic criteria for primary (n=11) and secondary SS (n=8) were studied. Seven healthy women served as controls. Computer assisted microscopy was employed to determine quantitatively the percentage of positive structures (acini, ducts, and interlobular connective tissue), the staining intensity, and the level of staining heterogeneity for 4 glycohistochemical probes including wheat germ agglutinin and concanavalin (Con A) as lectins, and mannose and N-acetylglucosamine as parts of neoglycoproteins. The data were evaluated by discriminant analysis.
The data strongly suggest that MBL related structures, if not MBL itself, could play distinct roles in the pathogenesis of primary versus secondary SS. Further, quantitative determination of the level of expression of D-mannose and N-acetylglucosamine and their respective binding sites in labial salivary gland acini offers a powerful diagnostic tool for distinguishing primary from secondary SS.
In SS labial salivary glands, determination of the level of acceptor sites for wheat germ agglutinin, Con A, D-mannose, and N-acetylglucosamine provides information on the roles played by glycoforms in SS. The methodology and data described in this paper should provide pathologists with objective diagnostic markers for SS. Our results should enhance the biological understanding of this pathology.
干燥综合征（SS）是一种自身免疫性外分泌腺病。甘露糖结合凝集素（MBL）是固有免疫系统的一种多能分子，参与自身免疫性疾病的发病机制。我们研究了MBL的特异性配体和MBL相关结构是否可作为SS病例的可靠标志物。
对19例符合原发性（n = 11）和继发性SS（n = 8）诊断标准的患者的唇腺进行研究。7名健康女性作为对照。采用计算机辅助显微镜定量测定4种糖组织化学探针（包括作为凝集素的麦胚凝集素和伴刀豆球蛋白A（Con A），以及作为新糖蛋白组成部分的甘露糖和N - 乙酰葡糖胺）的阳性结构（腺泡、导管和小叶间结缔组织）百分比、染色强度和染色异质性水平。数据通过判别分析进行评估。
数据强烈表明，MBL相关结构（若不是MBL本身）在原发性与继发性SS的发病机制中可能发挥不同作用。此外，定量测定唇腺腺泡中D - 甘露糖和N - 乙酰葡糖胺的表达水平及其各自的结合位点，为区分原发性和继发性SS提供了一种强大的诊断工具。
在SS唇腺中，测定麦胚凝集素、Con A、D - 甘露糖和N - 乙酰葡糖胺的受体位点水平，可提供糖型在SS中所起作用的信息。本文所述方法和数据应为病理学家提供SS的客观诊断标志物。我们的结果应能增强对这种病理状况的生物学理解。
