Turkcapar Nuran, Sak Serpil Dizbay, Saatci Mustafa, Duman Murat, Olmez Umit
Department of Clinical Immunology and Rheumatology, School of Medicine, Ankara University, Ankara, Turkey.
J Rheumatol. 2005 Jun;32(6):1063-70.
We investigated the relationship between clinical symptoms and the grade of histopathological damage and expression of adhesion molecules in salivary glands of patients with Sjögren's syndrome (SS).
We studied untreated and recently diagnosed patients with primary (n =20) and secondary SS [10 with SS and rheumatoid arthritis (RA); 10 with SS and systemic lupus erythematosus (SLE)] and 3 healthy controls. Salivary gland biopsies were performed in patients and controls and clinical data were obtained. Salivary gland biopsies were assessed for lymphocyte focus score and expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. In serum, antinuclear antibodies, rheumatoid factor, anti-Ro and anti-La antibodies, anti-alpha-fodrin IgA and IgG antibodies, and gamma-globulin concentrations were measured.
In salivary gland samples, ICAM-1 was expressed on vascular endothelial cells and lymphocyte foci, while VCAM-1 was expressed on vascular endothelial cells and follicular dendritic reticulin cells. There was a positive correlation between lymphocyte focus score and ICAM-1 expression (p < 0.05). We detected correlation between expression of ICAM-1 and VCAM-1, and the expression of VCAM-1 was significantly related to vasculitis (p < 0.05). The areas of E-selectin expression and the dispersion and severity of staining were not correlated with the focus score or with patients' clinical features (p > 0.05). There was no correlation between the staining and autoantibody positivity and gamma-globulin levels.
ICAM-1 may be important for lymphocyte recruitment and glandular damage and VCAM-1 may be important for the development of vasculitis in patients with SS.
我们研究了干燥综合征（SS）患者唾液腺的临床症状与组织病理学损伤程度及黏附分子表达之间的关系。
我们研究了未经治疗且近期诊断的原发性SS患者（n = 20）和继发性SS患者[10例合并类风湿关节炎（RA）的SS患者；10例合并系统性红斑狼疮（SLE）的SS患者]以及3名健康对照者。对患者和对照者进行唾液腺活检并获取临床数据。评估唾液腺活检的淋巴细胞灶评分以及细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和E-选择素的表达。检测血清中的抗核抗体、类风湿因子、抗Ro和抗La抗体、抗α- fodrin IgA和IgG抗体以及γ-球蛋白浓度。
在唾液腺样本中，ICAM-1表达于血管内皮细胞和淋巴细胞灶，而VCAM-1表达于血管内皮细胞和滤泡树突状网状细胞。淋巴细胞灶评分与ICAM-1表达呈正相关（p < 0.05）。我们检测到ICAM-1和VCAM-1的表达之间存在相关性，且VCAM-1的表达与血管炎显著相关（p < 0.05）。E-选择素表达区域以及染色的弥散和严重程度与灶评分或患者临床特征均无相关性（p > 0.05）。染色与自身抗体阳性及γ-球蛋白水平之间无相关性。
ICAM-1可能对淋巴细胞募集和腺体损伤起重要作用，而VCAM-1可能对SS患者血管炎的发生起重要作用。
