Buzoni-Gatel D, Debbabi H, Moretto M, Dimier-Poisson I H, Lepage A C, Bout D T, Kasper L H
Laboratoire Associé Institut National de la Recherche Agronomique d'Immunologie Parasitaire, Faculté de Pharmacie, Tours, France.
J Immunol. 1999 May 15;162(10):5846-52.
Toxoplasma gondii Ag-primed intraepithelial lymphocytes (IEL) from the mouse intestine have been shown to be protective against an lethal parasite challenge when adoptively transferred into recipient mice. In the present study, we observed that Ag-primed IEL traffic to the intestine of naive mice following i.v. administration. Primed and CD8beta+ IEL were the most efficient cells at homing to the host organ. In congenic mice, IEL migrated from intestine within several hours posttransfer. On Ag reexposure, the primed IEL return to the intestine where they enhance resistance as determined by reduction in the number of brain cysts. Treatment of recipient mice with anti-alpha4 and anti-alphaE Abs partially inhibited IEL intestinal homing. The Ab treatment dramatically impaired resistance to a subsequent oral infection. These finding indicate that lymphocyte homing is an important parameter in establishing long term immunity to recurrent infection with this parasite.
已证明，从小鼠肠道中提取的经弓形虫抗原致敏的上皮内淋巴细胞（IEL），在过继转移至受体小鼠体内时，可对致命的寄生虫攻击起到保护作用。在本研究中，我们观察到，经静脉注射给予抗原致敏的IEL后，它们会迁移至未接触过抗原的小鼠肠道。致敏的IEL和CD8β⁺ IEL是归巢至宿主器官效率最高的细胞。在同基因小鼠中，IEL在转移后数小时内从小肠迁移出来。再次接触抗原时，致敏的IEL会返回肠道，通过减少脑囊肿数量来增强抵抗力。用抗α4和抗αE抗体处理受体小鼠会部分抑制IEL向肠道的归巢。抗体处理显著削弱了对随后口服感染的抵抗力。这些发现表明，淋巴细胞归巢是建立对该寄生虫反复感染的长期免疫力的一个重要参数。
