Ignashov V G
Mol Biol (Mosk). 1976 Jul-Aug;10(4):682-5.
The treatment of denatured T4 phage DNA with antiserum for the DNA of this phage, containing antibodies against glucosylated 5-hydroxymethylcytosine, decreases the ability of DNA for renaturation. The greatest inhibiting activity is possessed by antiserum for T4 phage DNA irradiated with UV light, which contains antibodies not only against glucosylated 5-hydroxymethylcytosine, but also against the usual nitrogen bases. Antiserum against E. coli DNA, containing antibodies to the usual nitrogen bases, in equal dilutions with the antisera indicated above, shows less inhibitory activity on the renaturation of T4 phage DNA.
用针对该噬菌体DNA的抗血清处理变性的T4噬菌体DNA，该抗血清含有针对糖基化5-羟甲基胞嘧啶的抗体，会降低DNA复性的能力。对经紫外线照射的T4噬菌体DNA的抗血清具有最大的抑制活性，它不仅含有针对糖基化5-羟甲基胞嘧啶的抗体，还含有针对普通氮碱基的抗体。与上述抗血清等稀释度的针对大肠杆菌DNA的抗血清，其中含有针对普通氮碱基的抗体，对T4噬菌体DNA的复性显示出较小的抑制活性。
