Bolívar J, Iglesias C, Ortíz M, Goenechea L, Torres-Montaner A, Valdivia M M
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Cádiz, Spain.
Cell Mol Biol (Noisy-le-grand). 1999 Mar;45(2):277-84.
A human autoantigen (NOR-90) previously shown to be the upstream binding factor UBF, binds to the ribosomal RNA genes clustered at the nucleolus organizer regions (NORs). Truncated recombinant forms of hamster UBF were expressed in E. coli and it served to demonstrate that the DNA binding domains of UBF are major autoepitopes as shown by immunoblots analyses with a human autoimmune anti-NOR serum. Several monospecific antibodies to those recombinant truncated UBF polypeptides were generated as shown by immunofluorescence (IF) and Western blots. Immunoblots and IF studies using these anti-UBF sera in cell cultures of various vertebrates species from fish to mammals, indicate the conservation of the DNA binding domains of the ribosomal transcription factor during evolution.
一种先前被证明是上游结合因子UBF的人类自身抗原（NOR-90），可与聚集在核仁组织区（NORs）的核糖体RNA基因结合。仓鼠UBF的截短重组形式在大肠杆菌中表达，通过用人自身免疫性抗NOR血清进行免疫印迹分析表明，UBF的DNA结合结构域是主要的自身表位。通过免疫荧光（IF）和蛋白质印迹法显示，针对这些重组截短UBF多肽产生了几种单特异性抗体。在从鱼类到哺乳动物的各种脊椎动物细胞培养物中使用这些抗UBF血清进行免疫印迹和IF研究，表明核糖体转录因子的DNA结合结构域在进化过程中具有保守性。
