Zatsepina O V, Prusov A N, Semenov M V
Tsitologiia. 1995;37(1-2):137-44.
Serum P419 from a patient with rheumatoid arthritis with a high specificity immunolabeling nucleoli in various mammalian cells has been identified. On the Western blots of total cellular proteins or proteins extracted from isolated nucleoli it cross-reacted with a doublet of polypeptides of 97 and 94 kDa. That is why this serum has been concluded to recognize UBF, or RNA polymerase I-specific transcription initiation factor. It was shown that UBF remained bound to the nucleoli or nucleolus organizing regions (NORs) of mitotic chromosomes despite the level of rDNA transcription. Nevertheless, intranucleolar localization of UBF was dramatically changed after partial or complete block of rRNA synthesis. In pycnotic cells positive labeling was found within the whole nucleus and cytoplasm instead of nucleolus. In metaphase UBF molecules are unequally distributed between the particular NORs, whereas in anaphase they are uniformly allocated between the daughter cells.
已鉴定出一名类风湿性关节炎患者的血清P419，其在多种哺乳动物细胞中具有高特异性免疫标记核仁。在总细胞蛋白或从分离的核仁中提取的蛋白的Western印迹上，它与97 kDa和94 kDa的双条带多肽发生交叉反应。因此，得出结论，该血清识别UBF，即RNA聚合酶I特异性转录起始因子。结果表明，尽管rDNA转录水平不同，但UBF仍与有丝分裂染色体的核仁或核仁组织区（NORs）结合。然而，在rRNA合成部分或完全受阻后，UBF的核仁内定位发生了显著变化。在固缩细胞中，在整个细胞核和细胞质中发现阳性标记，而不是在核仁中。在中期，UBF分子在特定的NORs之间分布不均，而在后期，它们在子细胞之间均匀分配。
