Sánchez-Partida L G, Windsor D P, Eppleston J, Setchell B P, Maxwell W M
Department of Animal Science, University of Adelaide, Glen Osmond, Australia.
J Androl. 1999 Mar-Apr;20(2):280-8.
The fertility of ewes after artificial insemination and the relationship between fertility and motility characteristics assessed by a computerized motility analysis system were examined with ram semen frozen in diluents reported to improve postthaw motility. The percentages of motile and progressive spermatozoa were better when frozen in proline- or glycine betaine-containing or HEPES-based, rather than Tris-based, diluents (P < 0.01). The fertility of spermatozoa frozen in diluents containing proline or glycine betaine was slightly reduced, whereas when both compatible solutes were present, the reduction was more pronounced, in comparison with semen frozen in Tris- or HEPES-based diluents (9.5 versus 71.1 and 66.6%; P < 0.01). Fertility of frozen-thawed spermatozoa was higher after laparoscopic insemination than after cervical or transcervical insemination (P < 0.01). Similarly, higher fertility was obtained after cervical insemination with fresh than with frozen-thawed semen (32.4 versus 11.3%; P < 0.01). Furthermore, loss of embryos was lower after laparoscopic insemination of ewes with semen frozen in a Tris diluent than with semen frozen in proline diluents, in glycine betaine diluents, or in proline-plus-glycine betaine diluents (0.0 versus 26.0, 38.5, and 60.0%; P < 0.001). A wide variation in the postthaw percentage of motile (31.6-59.7%) and progressive (22.6-43.1%) spermatozoa and in the fertility of spermatozoa from individual rams was also observed after laparoscopic (29.2-59.7%) or cervical insemination (8.7-30.5%). Postthaw motility results from immediately after thawing and fertility results from experiments where intrauterine insemination was performed with semen frozen in proline- or glycine betaine-containing or HEPES- or Tris-based diluents were pooled and subjected to a pairwise correlation procedure. The correlation analysis showed relationships between some of the motility characteristics (P < 0.01), but there were no relationships between the motility characteristics and fertility.
研究了经人工授精后母羊的生育力，以及通过计算机化运动分析系统评估的生育力与精子活力特征之间的关系，所用的公羊精液冻存于据报道可提高解冻后活力的稀释液中。当精液冻存于含脯氨酸或甘氨酸甜菜碱的稀释液或基于HEPES的稀释液中，而非基于Tris的稀释液中时，活动精子和前进运动精子的百分比更高（P < 0.01）。与冻存于基于Tris或HEPES的稀释液中的精液相比，冻存于含脯氨酸或甘氨酸甜菜碱的稀释液中的精子生育力略有降低，而当两种相容性溶质都存在时，生育力降低更为明显（9.5%对71.1%和66.6%；P < 0.01）。腹腔镜授精后解冻精子的生育力高于宫颈或经宫颈授精后（P < 0.01）。同样，用新鲜精液进行宫颈授精后的生育力高于用解冻精液进行宫颈授精（32.4%对11.3%；P < 0.01）。此外，用冻存于Tris稀释液中的精液对母羊进行腹腔镜授精后胚胎损失低于用冻存于脯氨酸稀释液、甘氨酸甜菜碱稀释液或脯氨酸加甘氨酸甜菜碱稀释液中的精液进行腹腔镜授精（0.0%对26.0%、38.5%和60.0%；P < 0.001）。在腹腔镜授精（29.2% - 59.7%）或宫颈授精（8.7% - 30.5%）后，还观察到解冻后活动精子（31.6% - 59.7%）和前进运动精子（22.6% - 43.1%）的百分比以及来自个体公羊精子的生育力存在广泛差异。解冻后活力结果来自解冻后立即检测的数据，生育力结果来自用冻存于含脯氨酸或甘氨酸甜菜碱的稀释液或基于HEPES或Tris的稀释液中的精液进行子宫内授精的实验数据，将这些数据汇总并进行成对相关分析。相关分析显示了一些活力特征之间的关系（P < 0.01），但活力特征与生育力之间没有关系。
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