Fujii R, Ishikawa H, Mahmoud M S, Asaoku H, Kawano M M
Department of Immunohaematology, Yamaguchi University School of Medicine, Ube, Japan.
Br J Haematol. 1999 Apr;105(1):131-40.
Using three-colour phenotypic analysis, we detected five subpopulations of myeloma cells (CD38++) in the bone marrow mononuclear cells of human myeloma patients: MPC-1-CD45-CD49e-, MPC-1-CD45+CD49e-, MPC-1+CD45-CD49e-, MPC-1+CD45+CD49e- and MPC-1+CD45+CD49e+. Most of the myeloma cells did not express CD45 but a few MPC-1- immature myeloma cells and some MPC-1+ myeloma cells expressed CD45 and CD45RO but not CD45RA, whereas all of normal early plasma cells in the peripheral blood, lymph node plasma cells and bone marrow plasma cells expressed CD45 and CD45RA, CD45RB but not CD45RO. In order to clarify the biological character of these myeloma subpopulations, we examined the expression of Ki-67 antigen. Proliferating myeloma cells (Ki-67+) were found in the MPC-1- fractions and the MPC-1-CD45+ fractions rather than MPC-1-CD45- fractions. Next, in order to further clarify the biological difference of two immature subpopulations (MPC-1-CD45-CD49e- and MPC-1- CD45+CD49e-), determined cell viability and phenotypic change after culturing with interleukin 6 (IL-6) in vitro. In the presence of IL-6, MPC-1-CD45+ cells kept their viability more than MPC-1-CD45- cells and some MPC-1-CD45- cells could be converted to MPC-1-CD45+ cells. In conclusion, these data suggest that human myeloma cells are phenotypically subdivided into five subpopulations, and among these subpopulations MPC-1-CD45+CD49e- but not MPC-1-CD45-CD49e- immature cells contain proliferating cells in response to IL-6, and IL-6 can also induce expression of CD45 on MPC-1-CD45- subpopulation of immature myeloma cells.
利用三色表型分析，我们在人类骨髓瘤患者的骨髓单个核细胞中检测到骨髓瘤细胞（CD38++）的五个亚群：MPC-1-CD45-CD49e-、MPC-1-CD45+CD49e-、MPC-1+CD45-CD49e-、MPC-1+CD45+CD49e-和MPC-1+CD45+CD49e+。大多数骨髓瘤细胞不表达CD45，但少数MPC-1-未成熟骨髓瘤细胞和一些MPC-1+骨髓瘤细胞表达CD45和CD45RO，但不表达CD45RA，而外周血中的所有正常早期浆细胞、淋巴结浆细胞和骨髓浆细胞均表达CD45和CD45RA、CD45RB，但不表达CD45RO。为了阐明这些骨髓瘤亚群的生物学特性，我们检测了Ki-67抗原的表达。增殖的骨髓瘤细胞（Ki-67+）存在于MPC-1-亚群和MPC-1-CD45+亚群中，而不是MPC-1-CD45-亚群中。接下来，为了进一步阐明两个未成熟亚群（MPC-1-CD45-CD49e-和MPC-1-CD45+CD49e-）的生物学差异，我们在体外与白细胞介素6（IL-6）共培养后测定细胞活力和表型变化。在IL-6存在的情况下，MPC-1-CD45+细胞比MPC-1-CD45-细胞保持更高的活力，并且一些MPC-1-CD45-细胞可以转化为MPC-1-CD45+细胞。总之，这些数据表明人类骨髓瘤细胞在表型上可分为五个亚群，在这些亚群中，MPC-1-CD45+CD49e-而非MPC-1-CD45-CD,49e-未成熟细胞含有对IL-6有反应的增殖细胞，并且IL-6还可诱导未成熟骨髓瘤细胞的MPC-1-CD45-亚群表达CD45。
