Stein M S, Feik S A, Thomas C D, Clement J G, Wark J D
University of Melbourne Department of Medicine, Royal Melbourne Hospital, Australia.
J Bone Miner Res. 1999 Apr;14(4):624-32. doi: 10.1359/jbmr.1999.14.4.624.
The matrix of human cortical bone is arranged around a network of vascular spaces (hereafter referred to as "pores"). Our aim was to investigate age-related differences in human cortical porosity (total pore area divided by cortical bone area), pore size and number, and surface to volume ratios, while adjusting for sex, height, and weight. Ninety-six specimens of entire transverse sections of human femoral diaphysis, from subjects aged 21-92 years, were examined. We used our established automated image acquisition and analysis system which measures pores from entire sections of multiple specimens of bone. Over 400,000 pores were recorded. Results showed a greater porosity in older bone (p < 0.01) but marked variation in porosity for any given age. The cohort median, of the specimen medians, of pore cross-sectional area was 2050 microns 2. Older specimens did not have more pores than younger specimens but had a greater proportion of larger pores (p < 0.05) and greater intraspecimen variation in pore size (p < 0.001). The pore surface to bone matrix volume ratio was a median 2.3 mm2/mm3. This varied more than 4-fold between individuals but did not relate to age. No simple relationships were found between any of the measured parameters and either sex, height, or weight, even after adjustment for age. We conclude that the greater porosity in older specimens is due to greater pore size rather than a larger number of pores. Age, however, explains little of the inter-individual variation in the parameters studied.
人类皮质骨的基质围绕着血管间隙网络（以下简称“孔隙”）排列。我们的目的是研究人类皮质骨孔隙率（总孔隙面积除以皮质骨面积）、孔径和数量以及表面积与体积比的年龄相关差异，同时对性别、身高和体重进行校正。对96个来自21 - 92岁受试者的人股骨干完整横切片标本进行了检查。我们使用了已建立的自动图像采集和分析系统，该系统可测量多个骨标本整个切片中的孔隙。记录了超过400,000个孔隙。结果显示老年骨的孔隙率更高（p < 0.01），但在任何给定年龄，孔隙率都有显著变化。标本孔隙横截面积的队列中位数为2050平方微米。老年标本的孔隙数量并不比年轻标本多，但较大孔隙的比例更高（p < 0.05），且标本内孔隙大小的变化更大（p < 0.001）。孔隙表面积与骨基质体积比的中位数为2.3平方毫米/立方毫米。个体之间的这种比例变化超过4倍，但与年龄无关。即使在对年龄进行校正后，在所测量的参数与性别、身高或体重之间均未发现简单的关系。我们得出结论，老年标本中较高的孔隙率是由于孔径较大而非孔隙数量较多。然而，年龄对所研究参数的个体间差异解释甚少。
