[华氏肋突螈（两栖纲，有尾目）睾丸腺组织细胞中3-β-羟基类固醇脱氢酶活性的超微结构定位]
[Ultrastructural localization of 3-beta-hydroxysteroid dehydrogenase activity in the glandular tissue cells of the testis of Pleurodeles waltlii Michach. (Amphibia, Urodela)].
作者信息
Berchtold J P, Collenot G, Collenot A
出版信息
C R Acad Hebd Seances Acad Sci D. 1978 Sep 18;287(5):511-3.
PMID:102458
Abstract
With the potassium ferricyanide method, the localization of the sites of 3 beta-hydroxysteroid dehydrogenase activity has been investigated in the glandular tissue cells, previously fixed, of the Pleurodeles testis. An abundant copper ferrocyanide precipitate, the final reaction product, is observed in the vicinity of the external faces of the membranes of the smooth endoplasmic reticulum, or in contact with them. A very weak reaction occurs in mitochondrial cristae.
摘要
采用铁氰化钾法，对事先固定好的肋突螈睾丸腺组织细胞中3β - 羟基类固醇脱氢酶活性位点的定位进行了研究。在滑面内质网膜外表面附近或与之接触处观察到大量最终反应产物——亚铁氰化铜沉淀。线粒体嵴处发生非常微弱的反应。
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