Berchtold J P
C R Acad Hebd Seances Acad Sci D. 1976 Apr 26;282(16):1533-6.
Sections of Rat and Amphibian adrenocortical tissue fixed in a mixture of 1% formaldehyde and 0.25% glutaraldehyde, are incubated in a medium containing namely a 3 beta-hydroxysteroid (substrate), NAD, potassium ferricyanide (hydrogen acceptor) and copper sulfate. A hyaloplasmic copper ferrocyanide precipitate is observed in the immediate vicinity of the smooth endoplasmic reticulum membranes, or in close contact with them. This reaction, which does not occur in media lacking the substrate or containing cyanoketone, is a result of 3 beta-hydroxysteroid dehydrogenase activity. Problems concerning the precise localization of this enzyme are discussed.
将大鼠和两栖动物的肾上腺皮质组织切片固定于1%甲醛和0.25%戊二醛的混合液中，然后置于含有3β-羟基类固醇（底物）、NAD、铁氰化钾（氢受体）和硫酸铜的培养基中孵育。在滑面内质网膜的紧邻区域或与之紧密接触处观察到透明质铜亚铁氰化物沉淀。这种反应在缺乏底物或含有氰基酮的培养基中不发生，是3β-羟基类固醇脱氢酶活性的结果。文中讨论了关于该酶精确定位的问题。
