[Ultrastructural demonstration of 3 beta-hydroxysteroid dehydrogenase activity using potassium ferricyanide].
作者信息
Berchtold J P
出版信息
C R Acad Hebd Seances Acad Sci D. 1976 Apr 26;282(16):1533-6.
PMID:820453
Abstract
Sections of Rat and Amphibian adrenocortical tissue fixed in a mixture of 1% formaldehyde and 0.25% glutaraldehyde, are incubated in a medium containing namely a 3 beta-hydroxysteroid (substrate), NAD, potassium ferricyanide (hydrogen acceptor) and copper sulfate. A hyaloplasmic copper ferrocyanide precipitate is observed in the immediate vicinity of the smooth endoplasmic reticulum membranes, or in close contact with them. This reaction, which does not occur in media lacking the substrate or containing cyanoketone, is a result of 3 beta-hydroxysteroid dehydrogenase activity. Problems concerning the precise localization of this enzyme are discussed.
摘要
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引用本文的文献
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Ultrastructural demonstration of glucose-6-phosphate dehydrogenase activity in steroid-secreting cells.类固醇分泌细胞中葡萄糖-6-磷酸脱氢酶活性的超微结构显示
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2
The ultrastructural localization of the enzymes related to steroid hormone metabolism in the guinea-pig testis.豚鼠睾丸中与甾体激素代谢相关酶的超微结构定位
Histochem J. 1979 Jan;11(1):51-71. doi: 10.1007/BF01041265.
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