Kagawa H, Nagahama Y
Cell Tissue Res. 1980;212(2):225-31. doi: 10.1007/BF00233957.
The ultrastructural localization of the enzyme delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) was studied in the goldfish interrenal cells by using the potassium ferricyanide method. Immersion fixation in a mixture of 1% paraformaldehyde and 0.25% glutaraldehyde results in a consistently good ultrastructural preservation and enzyme localization. A precipitate of copper ferrocyanide indicating the localization of 3 beta-HSD activity was observed in close vicinity to the smooth endoplasmic reticulum or in contact with the outer surface of its membrane. A small number of precipitated grains were also found in the lumen of mitochondrial cristae. Addition of phenazine methosulfate increased the intensity of the reaction without changing the localization of the copper ferrocyanide grains. The findings suggest that the outer surface of the smooth endoplasmic reticulum is the major site of 3 beta-HSD activity in goldfish interrenal cells.
采用铁氰化钾法研究了金鱼肾上腺细胞中δ5-3β-羟基类固醇脱氢酶（3β-HSD）的超微结构定位。将样品浸入1%多聚甲醛和0.25%戊二醛的混合液中固定，可始终保持良好的超微结构保存和酶定位。在光滑内质网附近或与其膜外表面接触处观察到指示3β-HSD活性定位的亚铁氰化铜沉淀。在线粒体嵴的腔内也发现少量沉淀颗粒。加入吩嗪硫酸甲酯可增加反应强度，而不改变亚铁氰化铜颗粒的定位。这些发现表明，光滑内质网的外表面是金鱼肾上腺细胞中3β-HSD活性的主要部位。
