Molchanov M I, Trusova V M, Shaposhnikov G L
Biokhimiia. 1976 May;41(5):926-32.
The biosynthesis of membrane proteins in maize plastids at different stages of differentiation of the chloroplast lamellar system was studied. Prolamellar and lamellar system preparations were isolated from maize plastids, disintegrated by osmotic shock under hypotonic conditions. Changes in the amino acid composition of 14C membrane proteins were observed at all stages of chloroplast ultrastructure formation. The maximal level of the apolar amino acids was observed in the membrane fraction of chloroplasts. Washed membranes from maize proplastids and chloroplasts can be resolved into at least 14 protein bands on formic acid--urea polyacrylamide gel. It is pointed out that biogenesis process leads to the increase of lipophylic protein content in the chloroplast lamellae fraction.
研究了叶绿体片层系统不同分化阶段玉米质体中膜蛋白的生物合成。从玉米质体中分离出原片层体和片层系统制剂,在低渗条件下通过渗透休克使其解体。在叶绿体超微结构形成的所有阶段都观察到了14C膜蛋白氨基酸组成的变化。在叶绿体的膜部分观察到非极性氨基酸的最高水平。玉米原质体和叶绿体的洗涤膜在甲酸-尿素聚丙烯酰胺凝胶上可分离成至少14条蛋白带。指出生物发生过程导致叶绿体片层部分中亲脂性蛋白质含量增加。