[The nature of peptides firmly associated with the protein part of membrane lipoproteins in chloroplast biogenesis].

The biosynthesis of membrane lipoproteins in maize plastids at different stages of differentiation of the chloroplast lamellar system was studied. Prolamellar and lamellar system preparations were isolated from maize plastids and been or kidney bean chloroplasts, disintegrated by osmotic shock. Chloroplast lipoproteins are membraneous proteins of chloroplast lamellar systems. Peptids, lipids and carbohydrates "firmly" associated with the protein part of membrane lipoproteins were found at all stages of chloroplast ultrastructure formation. Incorporation of C14 amino acid increased with the time of incubation in "firmly" lipoprotein--bound peptids and in the polypeptid part of lipoproteins. Puromycin but not actidion D inhibited amino acid incorporation into the peptids and polypeptids of chloroplasts in vitro experiments. The maximal level of C14-peptids "firmly" associated with the protein part of membrane lipoproteins was observed in the membrane fraction of proplastids in vivo. It is pointed out that biogenesis process leads to the decrease of content of "firmly" lipoprotein--bound peptids in the chloroplast lamellae fraction.