Neary J T, Nakamura C, Maloof F, Soodak M
Clin Chim Acta. 1978 Nov 15;90(1):61-70. doi: 10.1016/0009-8981(78)90084-0.
A rapid and sensitive method has been developed to assay thyrotropin releasing hormone (TRH)-degrading activity in serum. In this assay, the formation of proline, a major serum degradation product of TRH (pGlu-His-Pro-NH2), is measured. The procedure is based on the finding that proline can be readily separated from TRH with Dowex 50 by a batchwise procedure in a test tube.
已开发出一种快速灵敏的方法来测定血清中促甲状腺激素释放激素（TRH）的降解活性。在该测定中，测量TRH（焦谷氨酸-组氨酸-脯氨酸-酰胺，pGlu-His-Pro-NH2）的主要血清降解产物脯氨酸的生成。该方法基于以下发现：在试管中通过分批操作，脯氨酸可利用Dowex 50轻松地与TRH分离。
