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来自碳氢化合物的碳水化合物。II. 在正十六烷上生长的酵母细胞中的游离糖和结合糖。

Carbohydrates from hydrocarbons. II. Free and bound sugars from yeast cells grown on n-hexadecane.

作者信息

Jwanny E W, Hussein M M

出版信息

Acta Biol Acad Sci Hung. 1976;27(2-3):101-6.

PMID:1025983
Abstract

Candida lipolytica (strain 10) was grown on an n-hexadecane medium with and without yeast extract. The harvested dry cells were weighed at various stages of growth. The free sugars from the cultures were obtained by Soxhlet extraction with 85% ethyl alcohol. Further qualitative and quantitative analyses of free monosaccharides in the concentrated alcoholic extracts were made by paper chromatography. Glucose was the only free monosaccharide that could be identified at various stages of growth. The chromatographic analysis of the acid-hydrolyzed yeast cells indicated the presence of glucose and mannose as dominant bound sugars; galactose and xylose were present in minor quantities. In the harvested dry cells from the yeast extract-containing medium, in general, greater amounts of bound sugars were present.

摘要

解脂假丝酵母(菌株10)在含有和不含有酵母提取物的正十六烷培养基上生长。在生长的各个阶段对收获的干细胞进行称重。通过用85%乙醇进行索氏提取获得培养物中的游离糖。通过纸色谱法对浓缩酒精提取物中的游离单糖进行进一步的定性和定量分析。葡萄糖是在生长的各个阶段都能鉴定出的唯一游离单糖。酸水解酵母细胞的色谱分析表明,葡萄糖和甘露糖是主要的结合糖;半乳糖和木糖含量较少。一般来说,在含有酵母提取物的培养基中收获的干细胞中,结合糖的含量更高。

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Acta Biol Acad Sci Hung. 1976;27(2-3):101-6.
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