Ilchenko A P, Tyurin V S, Dmitriev V V
Prikl Biokhim Mikrobiol. 1975 Mar-Apr;11(2):162-6.
A method for isolating intact mitochondria from Torulopsis candida grown on glucose and hexadecane was developed. The isolated mitochondria were capable of oxidizing substrates of the di- and tricarboxylic acid cycle and of showing Chance respiration control. The morphological examination of the fractions showed that the major portion of mitochondria from yeast grown on glucose were in the condensed form whereas mitochondria from yeast grown on hexadecane were in the orthodoxal form.
开发了一种从在葡萄糖和十六烷上生长的假丝酵母中分离完整线粒体的方法。分离出的线粒体能够氧化二羧酸和三羧酸循环的底物,并表现出Chance呼吸控制。对各组分的形态学检查表明,在葡萄糖上生长的酵母中的线粒体大部分呈浓缩形式,而在十六烷上生长的酵母中的线粒体呈正统形式。