Patterson R, Suszko I M, Bacal E, Zeiss C R, Kelly J F, Pruzansky J J
J Allergy Clin Immunol. 1979 Jan;63(1):47-50. doi: 10.1016/0091-6749(79)90161-1.
Polymerized ragweed antigens of different molecular weight ranges were studied to determine if the degree of allergenicity was dependent on molecular size of the polymers. In this study, allergenicity is defined as the ability to elict IgE-mediated skin reactivity. Ragweed antigen treated with glutaraldehyde was fractionated into one preparation with a molecular weight range of 200,000 to 20,000,000 and another with molecular weights less than 200,000. Except for the difference in molecular sizes of the two molecular sizes of the two preparations, all ragweed polymers had been treated in an identical fashion. The low molecular weight fraction had cutaneous endpoint reactivity greater than 10(5) to 10(8) times that of the high molecular weight materials. In these and other studies, immunogenicity defined as the ability to induce an IgG antibody response was retained by the high molecular weight material. The results are consitent with the hypothesis that allergenicity decreases as the molecular weight of the polymerized allergen increases.
对不同分子量范围的聚合豚草抗原进行了研究，以确定变应原性程度是否取决于聚合物的分子大小。在本研究中，变应原性定义为引发IgE介导的皮肤反应性的能力。用戊二醛处理的豚草抗原被分级分离成一种分子量范围为200,000至20,000,000的制剂和另一种分子量小于200,000的制剂。除了两种制剂的分子大小不同外，所有豚草聚合物均以相同方式处理。低分子量级分的皮肤终点反应性比高分子量材料高10(5)至10(8)倍。在这些及其他研究中，高分子量材料保留了定义为诱导IgG抗体反应能力的免疫原性。这些结果与以下假设一致，即随着聚合变应原分子量的增加，变应原性降低。
