使用液氮冷冻红细胞结合相应病毒抗原进行甲型流感、麻疹和风疹抗体的凝胶内溶血（HIG）试验。
Hemolysis-in-gel (HIG) test for antibodies to influenza A, measles and mumps using liquid nitrogen freezed erythrocytes coupled with the respective viral antigen.
作者信息
Wesslén L
出版信息
J Immunol Methods. 1978;24(1-2):1-8. doi: 10.1016/0022-1759(78)90079-0.
PMID:102699
Abstract
A drawback with the hemolysis-in-gel test is the constant need for fresh erythrocytes which must be treated with virus before incorporation in the gel. This problem was overcome by freezing small droplets containing erythrocytes to which antigen had been attached. The droplets were stored at -70 degrees C or in liquid nitrogen. This modification was applied in detecting antibodies to influenza, measles and mumps viruses and the results were shown to equal those obtained with fresh erythrocytes.
摘要
凝胶内溶血试验的一个缺点是持续需要新鲜红细胞，在将其加入凝胶之前必须用病毒进行处理。通过冷冻含有已附着抗原的红细胞的小液滴，这个问题得到了解决。这些液滴保存在-70℃或液氮中。这种改进方法应用于检测针对流感病毒、麻疹病毒和腮腺炎病毒的抗体，结果显示与使用新鲜红细胞所获得的结果相当。
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