Heide K G, Steinmann J
Zentralbl Bakteriol Orig A. 1978 Jan;240(1):1-6.
Different kinds of erythrocytes (human, pigeon, chicken and sheep) and complement (guinea pig, rabbit and rat) were tested in rubella single radial hemolysis-test (SRHT, hemolysis-in-gel = HIG-test) in order to detect IgG and IgM antibodies. Experiments showed that sera which were used as complement, contained antibodies against different kinds of erythrocytes. If complement from rabbit and rat should be used, an adsorption will be necessary. For serological surveillance sheep and chicken erythrocytes in connection with guinea pig serum as complement were best suited. Positive results were also obtained with pigeon erythrocytes whereas human RBC gave negative reactions. A detection of IgM antibodies in the SRHT was not possible.
为检测IgG和IgM抗体，在风疹单向辐射溶血试验（SRHT，凝胶内溶血=HIG试验）中对不同种类的红细胞（人、鸽、鸡和绵羊）以及补体（豚鼠、兔和大鼠）进行了测试。实验表明，用作补体的血清含有针对不同种类红细胞的抗体。如果要使用兔和大鼠的补体，则需要进行吸附处理。对于血清学监测，绵羊和鸡的红细胞与豚鼠血清作为补体最为合适。使用鸽红细胞也获得了阳性结果，而人红细胞则产生阴性反应。在SRHT中无法检测到IgM抗体。
