Redgrave T G, Vakakis N
Aust J Exp Biol Med Sci. 1976 Dec;54(6):519-25. doi: 10.1038/icb.1976.53.
These experiments were designed to test the hypothesis that the vitamin A fat-storage cell removes chylomicron remnant cholesterol from hepatic portal venous blood; A modified Ficoll density gradient ultracentrifugation procedure was used to isolate from rat liver cellular fractions that were enriched in vitamin A. In rats fed a normal diet and in rats fed excess vitamin A isolated hepatocytes were fractionated 15 min after the intravenous injection of chylomicrons labelled in vivo with radioactive cholesterol. The results showed that cholesterol radioactivity was not concentrated in the vitamin A enriched cellular fractions, so it was concluded that the vitamin A fat-storage cell is not implicated in clearance of chylomicron remnants by the liver.
维生素A脂肪储存细胞可从肝门静脉血中清除乳糜微粒残余胆固醇；采用改良的Ficoll密度梯度超速离心法从大鼠肝脏中分离出富含维生素A的细胞组分。给正常饮食的大鼠和过量摄入维生素A的大鼠静脉注射体内用放射性胆固醇标记的乳糜微粒15分钟后，将分离的肝细胞进行分级分离。结果显示，胆固醇放射性并未在富含维生素A的细胞组分中富集，因此得出结论：维生素A脂肪储存细胞与肝脏清除乳糜微粒残余物无关。
