Demidkina T V, Torchinskiĭ Iu M
Biokhimiia. 1976 Jul;41(6):1132-5.
Homogeneous cytosolic aspartate aminotransferase was prepared from chicken muscle. The purification procedure involves heat and ammonium sulfate fractionation, chromatography on ion-exchage cellulose CM-52 and crystallization of the enzyme. A comparison of some properties of aspartate aminotransferases from chicken skeletal muscle and heart has been made. Both enzymes were found identical in terms of their electrophoretic mobility, isoelectric point, pyridoxal phosphate content and the amount of SH-groups.
从鸡肌肉中制备了均一的胞质天冬氨酸氨基转移酶。纯化步骤包括加热、硫酸铵分级分离、在离子交换纤维素CM - 52上进行色谱分离以及该酶的结晶。对鸡骨骼肌和心脏中的天冬氨酸氨基转移酶的某些特性进行了比较。发现这两种酶在电泳迁移率、等电点、磷酸吡哆醛含量和巯基数量方面是相同的。