Imperial S, Busquets M, Cortés A, Bozal J
Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
Prep Biochem. 1989;19(2):113-27. doi: 10.1080/10826068908544902.
Cytosolic aspartate aminotransferases from chicken heart, liver, spleen, skeletal muscle and breast muscle differed in number of their molecular forms, detected by polyacrylamide gel electrophoresis and specific staining. The number of molecular forms varied from tissue to tissue but the electrophoretic mobilities of a given form in all tissues were analogous. Within a single tissue most of the enzyme activity was present as the lowest-running band (alpha form) and the rest was distributed in minor bands termed (B,tau, alpha and epsilon forms). We report a method for the purification of cytosolic aspartate aminotransferases from various chicken tissues. The procedure can be carried out in one week and allows the obtention of isolated molecular forms of the enzyme, independently of the tissue under study. Separation of multiple forms was also achieved by chromatofocusing. The isoelectric points determined by this method for a given form in all five tissues were analogous and differed from those of the molecular forms of the enzyme from other origins. An Mr of 100,000 was obtained for all molecular forms of the five chicken tissues studied.
通过聚丙烯酰胺凝胶电泳和特异性染色检测发现，鸡心脏、肝脏、脾脏、骨骼肌和胸肌中的胞质天冬氨酸转氨酶分子形式数量存在差异。分子形式的数量因组织而异，但给定形式在所有组织中的电泳迁移率相似。在单个组织中，大部分酶活性以迁移率最低的条带（α形式）存在，其余分布在称为（B、τ、α和ε形式）的次要条带中。我们报告了一种从鸡的各种组织中纯化胞质天冬氨酸转氨酶的方法。该过程可在一周内完成，且能获得该酶的分离分子形式，与所研究的组织无关。通过色谱聚焦也实现了多种形式的分离。用该方法测定的所有五种组织中给定形式的等电点相似，且与其他来源的该酶分子形式的等电点不同。在所研究的鸡的五种组织的所有分子形式中，获得的分子量均为100,000。
