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对铜绿假单胞菌菌体抗原和鞭毛抗原的抗体反应(作者译)

[Antibody response to somatic and flagellar antigens of Pseudomonas aeruginosa (author's transl)].

作者信息

Ansorg R, Schmitt W, Schwerk V

出版信息

Med Microbiol Immunol. 1978 Oct 20;165(3):181-9. doi: 10.1007/BF02123175.

Abstract

Using the indirect fluorescent antibody technique, the production of antiflagellar and antisomatic antibodies in rabbits immunized with different P. aeruginosa strains is measured. After 6 injections of formalinized cultures in graded doses over 3 weeks, the antiflagellar antibodies reach the peak in the 4th to 5th week, followed by a rapid fall. The antisomatic antibodies reacting with native and boiled cells show a plateau-shaped maximum from the 3rd to 5th/6th week and fell slightly up to the 11th week. A significant dissociation of antiflagellar and antisomatic antibodies, however, is not observed. The antisomatic antibodies are less absorbed by protein A (Staph. aureus) than are the antiflagellar antibodies.

摘要

采用间接荧光抗体技术,测定用不同铜绿假单胞菌菌株免疫的家兔中抗鞭毛抗体和抗菌体抗体的产生情况。在3周内分6次注射不同剂量的福尔马林固定培养物后,抗鞭毛抗体在第4至5周达到峰值,随后迅速下降。与天然细胞和煮沸细胞反应的抗菌体抗体在第3至5/6周呈现平台状最大值,并在第11周前略有下降。然而,未观察到抗鞭毛抗体和抗菌体抗体的明显解离。抗菌体抗体比抗鞭毛抗体更不易被蛋白A(金黄色葡萄球菌)吸收。

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